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CoraLite®594-conjugated TRIM2 Monoclonal antibody

TRIM2 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H9C2

Cat no : CL594-67342

Print datasheet

Synonyms

KIAA0517, RING finger protein 86, RNF86, TRIM2, tripartite motif containing 2

验证数据展示

  • IF Staining of mouse brain using CL594-67342

    IF Staining of mouse brain using CL594-67342

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 TRIM2 antibody (CL594-67342, Clone: 1H9C2 ) at dilution of 1:200.

  • IF Staining of mouse brain using CL594-67342

    IF Staining of mouse brain using CL594-67342

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 TRIM2 antibody (CL594-67342, Clone: 1H9C2 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 67342-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67342 targets TRIM2 in IF-P applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications IF-P Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen TRIM2 fusion protein Ag14637 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tripartite motif-containing 2
Synonyms KIAA0517, RING finger protein 86, RNF86, TRIM2, tripartite motif containing 2
Calculated Molecular Weight 744 aa, 82 kDa
Observed Molecular Weight 70-85 kDa
GenBank Accession NumberBC011052
Gene Symbol TRIM2
Gene ID (NCBI) 23321
RRIDAB_2920118
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9C040
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tripartite motif-containing 2(TRIM2) is an E3 ubiquitin ligase which directs proteasome-mediated degradation of target proteins by the ubiquitination of NEFL and phosphorylation of BCL2L11. This protein contains an N-terminal RING domain, followed by a B-box-2 domain, a coiled-coil region, and 6 C-terminal NHL repeats. TRIM2 involves in the apoptotic response to ischemia via directing degradation of BIM protein, thus has a role in neuroprotection.

实验方案

Product Specific Protocols
IF protocol for CL594 TRIM2 antibody CL594-67342Download protocol
Standard Protocols
Click here to view our Standard Protocols