CoraLite® Plus 488-conjugated TRIM21 Recombinant antibody
TRIM21 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240491B2
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | A549 cells |
Planning an IHC experiment? We recommend our IHCeasy TRIM21 Ready-To-Use IHC Kit. TRIM21 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83530-3-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of CL488-83530-3 is TRIM21 Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Tested Reactivity | human |
| Immunogen | TRIM21 fusion protein Ag28377 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | tripartite motif-containing 21 |
| Synonyms | RNF 81, RING finger protein 81, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM21, 52 kDa ribonucleoprotein autoantigen Ro/SS-A |
| Calculated Molecular Weight | 475 aa, 54 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC010861 |
| Gene Symbol | TRIM21 |
| Gene ID (NCBI) | 6737 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P19474 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM3 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules.
TRIM21 exists various isoforms and range molecular weight of isoforms is about 42-45kDa and 49-54 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 TRIM21 antibody CL488-83530-3 | Download protocol |
| FC protocol for CL Plus 488 TRIM21 antibody CL488-83530-3 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |