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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TRIM24 Polyclonal antibody

TRIM24 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-14208

Print datasheet

Synonyms

TRIM24, tripartite motif containing 24, Tripartite motif containing protein 24, Tripartite motif-containing protein 24, E3 ubiquitin-protein ligase TRIM24, EC:2.3.2.27, RING-type E3 ubiquitin transferase TIF1-alpha, TIF1, TIF1A, TIF1-alpha, Transcription intermediary factor 1-alpha, TRIM, TRIM 24

验证数据展示

  • IF Staining of HeLa using CL488-14208

    IF Staining of HeLa using CL488-14208

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 TRIM24 antibody (CL488-14208) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-14208

    FC experiment of HeLa using CL488-14208

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated TRIM24 Polyclonal antibody (CL488-14208)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TRIM24 Ready-To-Use IHC Kit. TRIM24 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14208-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14208 targets TRIM24 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen TRIM24 fusion protein Ag5417 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tripartite motif-containing 24
Synonyms TRIM24, tripartite motif containing 24, Tripartite motif containing protein 24, Tripartite motif-containing protein 24, E3 ubiquitin-protein ligase TRIM24, EC:2.3.2.27, RING-type E3 ubiquitin transferase TIF1-alpha, TIF1, TIF1A, TIF1-alpha, Transcription intermediary factor 1-alpha, TRIM, TRIM 24
Calculated Molecular Weight 117 kDa
Observed Molecular Weight 117 kDa
GenBank Accession NumberBC028689
Gene Symbol TRIM24
Gene ID (NCBI) 8805
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15164
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM24, also named as RNF82, TIF1 and TIF1A, interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors, it requires the presence of estradiol. It is a regulation protein of P53. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) wich is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TRIM24 antibody CL488-14208Download protocol
FC protocol for CL Plus 488 TRIM24 antibody CL488-14208Download protocol
Standard Protocols
Click here to view our Standard Protocols