验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, K-562 cells, BxPC-3 cells, mouse skeletal muscle tissue, HeLa cells, NIH/3T3 cells, C6 cells |
| Positive IP detected in | K-562 cells |
| Positive IF/ICC detected in | HeLa cells, BxPC-3 cells |
Planning an IHC experiment? We recommend our IHCeasy TRIP10 Ready-To-Use IHC Kit. TRIP10 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10798-1-AP targets TRIP10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | TRIP10 fusion protein Ag1251 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | thyroid hormone receptor interactor 10 |
| 别名 | TRIP-10, TR-interacting protein 10, Thyroid receptor-interacting protein 10, hSTP, CIP4 |
| 计算分子量 | 63 kDa |
| 观测分子量 | 75-85 kDa |
| GenBank蛋白编号 | BC013002 |
| 基因名称 | TRIP10 |
| Gene ID (NCBI) | 9322 |
| RRID | AB_2209248 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15642 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRIP10 (thyroid hormone receptor interactor 10), also known as CIP4 (Cdc42-interacting protein 4), is a novel adaptor protein which may be invloved in plasma membrane remodeling. CIP4 has been reported to negatively regulate glucose uptake by promoting GLUT4 endocytosis in muscle cells. At least five splice variants for human CIP4 have been identified, including CIP4a (CIP4), CIP4b (FELIC), CIP4c, CIPh (CIP4/2). Western blot analysis usually demonstrates a major band around 75 kDa and a weaker band about 85 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIP10 antibody 10798-1-AP | Download protocol |
| IF protocol for TRIP10 antibody 10798-1-AP | Download protocol |
| IP protocol for TRIP10 antibody 10798-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Mechanical loading of intraluminal pressure mediates wound angiogenesis by regulating the TOCA family of F-BAR proteins. | ||
J Exp Clin Cancer Res CDC42-interacting protein 4 promotes metastasis of nasopharyngeal carcinoma by mediating invadopodia formation and activating EGFR signaling.
|
