验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy TRIP10 Ready-To-Use IHC Kit. TRIP10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10798-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-10798 targets TRIP10 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | TRIP10 fusion protein Ag1251 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | thyroid hormone receptor interactor 10 |
| 别名 | Cdc42 interacting protein 4, CIP4, HSTP, Protein Felic, Salt tolerant protein, STOT, STP, TR interacting protein 10, TRIP 10, TRIP10 |
| 计算分子量 | 63 kDa |
| 观测分子量 | 75-85 kDa |
| GenBank蛋白编号 | BC013002 |
| 基因名称 | TRIP10 |
| Gene ID (NCBI) | 9322 |
| RRID | AB_3672466 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15642 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRIP10 (thyroid hormone receptor interactor 10), also known as CIP4 (Cdc42-interacting protein 4), is a novel adaptor protein which may be invloved in plasma membrane remodeling. CIP4 has been reported to negatively regulate glucose uptake by promoting GLUT4 endocytosis in muscle cells. At least five splice variants for human CIP4 have been identified, including CIP4a (CIP4), CIP4b (FELIC), CIP4c, CIPh (CIP4/2). Western blot analysis usually demonstrates a major band around 75 kDa and a weaker band about 85 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 TRIP10 antibody CL488-10798 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |