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  • Featured Product
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Tuberin/TSC2 Monoclonal antibody

Tuberin/TSC2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A10B2

Cat no : 68380-1-Ig

Print datasheet

Synonyms

FLJ43106, LAM, TSC2, TSC4, Tuberin, tuberous sclerosis 2, Tuberous sclerosis 2 protein

验证数据展示

  • WB analysis using 68380-1-Ig

    WB analysis using 68380-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68380-1-Ig

    WB analysis of HeLa using 68380-1-Ig

    WB result of Tuberin/TSC2 antibody (68380-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Tuberin/TSC2 transfected HeLa cells.

  • IF Staining of HEK-293 using 68380-1-Ig

    IF Staining of HEK-293 using 68380-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TSC2 antibody (68380-1-Ig, Clone: 1A10B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • WB analysis of MCF-7 using 68380-1-Ig

    WB analysis of MCF-7 using 68380-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 68380-1-Ig

    WB analysis of PC-3 using 68380-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 68380-1-Ig

    WB analysis of NIH/3T3 using 68380-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 68380-1-Ig

    WB analysis of HSC-T6 using 68380-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, PC-3 cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, U2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68380-1-Ig targets Tuberin/TSC2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, Mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tuberous sclerosis 2
Synonyms FLJ43106, LAM, TSC2, TSC4, Tuberin, tuberous sclerosis 2, Tuberous sclerosis 2 protein
Calculated Molecular Weight 1807 aa, 201 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberBC150300
Gene Symbol TSC2
Gene ID (NCBI) 7249
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49815
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling.

实验方案

Product Specific Protocols
WB protocol for Tuberin/TSC2 antibody 68380-1-IgDownload protocol
IF protocol for Tuberin/TSC2 antibody 68380-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols