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验证数据展示

  • WB analysis using 68380-1-Ig

    WB analysis using 68380-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68380-1-Ig

    WB analysis of HeLa using 68380-1-Ig

    WB result of Tuberin/TSC2 antibody (68380-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Tuberin/TSC2 transfected HeLa cells.

  • IF Staining of HEK-293 using 68380-1-Ig

    IF Staining of HEK-293 using 68380-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TSC2 antibody (68380-1-Ig, Clone: 1A10B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • WB analysis of MCF-7 using 68380-1-Ig

    WB analysis of MCF-7 using 68380-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 68380-1-Ig

    WB analysis of PC-3 using 68380-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 68380-1-Ig

    WB analysis of NIH/3T3 using 68380-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 68380-1-Ig

    WB analysis of HSC-T6 using 68380-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 68380-1-Ig

    IHC staining of rat brain using 68380-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TSC2 antibody included
  • IHC staining of rat brain using 68380-1-Ig

    IHC staining of rat brain using 68380-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TSC2 antibody included
  • IHC staining of human lung cancer using 68380-1-Ig

    IHC staining of human lung cancer using 68380-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TSC2 antibody included
  • Featured Product
  • KD/KO验证

Tuberin/TSC2 Monoclonal antibody

Tuberin/TSC2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68380-1-Ig

产品说明书

CloneNo. 1A10B2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

TSC2, Tuberin, tuberous sclerosis 2, TSC4, 1A10B2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, PC-3 cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, U2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inrat brain tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy TSC2 Ready-To-Use IHC Kit. TSC2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68380-1-Ig targets Tuberin/TSC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tuberous sclerosis 2
别名 TSC2, Tuberin, tuberous sclerosis 2, TSC4, 1A10B2
计算分子量 1807 aa, 201 kDa
观测分子量 200 kDa
GenBank蛋白编号BC150300
基因名称 TSC2
Gene ID (NCBI) 7249
RRIDAB_3085098
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP49815
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling.

实验方案

Product Specific Protocols
WB protocol for Tuberin/TSC2 antibody 68380-1-IgDownload protocol
IHC protocol for Tuberin/TSC2 antibody 68380-1-IgDownload protocol
IF protocol for Tuberin/TSC2 antibody 68380-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols