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  • Featured Product
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Tuberin/TSC2 Monoclonal antibody

Tuberin/TSC2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A10B2

Cat no : 68380-1-Ig

Print datasheet

Synonyms

TSC2, Tuberin, tuberous sclerosis 2, TSC4, 1A10B2

验证数据展示

  • WB analysis using 68380-1-Ig

    WB analysis using 68380-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68380-1-Ig

    WB analysis of HeLa using 68380-1-Ig

    WB result of Tuberin/TSC2 antibody (68380-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Tuberin/TSC2 transfected HeLa cells.

  • IF Staining of HEK-293 using 68380-1-Ig

    IF Staining of HEK-293 using 68380-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TSC2 antibody (68380-1-Ig, Clone: 1A10B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • WB analysis of MCF-7 using 68380-1-Ig

    WB analysis of MCF-7 using 68380-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 68380-1-Ig

    WB analysis of PC-3 using 68380-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 68380-1-Ig

    WB analysis of NIH/3T3 using 68380-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 68380-1-Ig

    WB analysis of HSC-T6 using 68380-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 68380-1-Ig

    IHC staining of rat brain using 68380-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TSC2 antibody included
  • IHC staining of rat brain using 68380-1-Ig

    IHC staining of rat brain using 68380-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TSC2 antibody included
  • IHC staining of human lung cancer using 68380-1-Ig

    IHC staining of human lung cancer using 68380-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TSC2 antibody included

经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, PC-3 cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, U2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inrat brain tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy TSC2 Ready-To-Use IHC Kit. TSC2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68380-1-Ig targets Tuberin/TSC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tuberous sclerosis 2
Synonyms TSC2, Tuberin, tuberous sclerosis 2, TSC4, 1A10B2
Calculated Molecular Weight 1807 aa, 201 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberBC150300
Gene Symbol TSC2
Gene ID (NCBI) 7249
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49815
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling.

实验方案

Product Specific Protocols
WB protocol for Tuberin/TSC2 antibody 68380-1-IgDownload protocol
IHC protocol for Tuberin/TSC2 antibody 68380-1-IgDownload protocol
IF protocol for Tuberin/TSC2 antibody 68380-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols