CoraLite® Plus 488-conjugated TSC2 Monoclonal antibody
TSC2 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1A10B2
验证数据展示
经过测试的应用
Positive IF detected in | HEK-293 cells |
For other applications, we recommend the unconjugated version of this antibody, 68380-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-68380 targets TSC2 in IF applications and shows reactivity with Human, Mouse samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human, Mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | tuberous sclerosis 2 |
Synonyms | FLJ43106, LAM, TSC2, TSC4, Tuberin, tuberous sclerosis 2, Tuberous sclerosis 2 protein |
Calculated Molecular Weight | 1807 aa, 201 kDa |
Observed Molecular Weight | 200 kDa |
GenBank Accession Number | BC150300 |
Gene Symbol | TSC2 |
Gene ID (NCBI) | 7249 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P49815 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling.
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 TSC2 antibody CL488-68380 | Download protocol |
FC protocol for CL Plus 488 TSC2 antibody CL488-68380 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |