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验证数据展示

  • IF Staining of HEK-293 using CL488-68380

    IF Staining of HEK-293 using CL488-68380

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 TSC2 antibody (CL488-68380, Clone: 1A10B2 ) at dilution of 1:200.

  • FC experiment of HEK-293 using CL488-68380

    FC experiment of HEK-293 using CL488-68380

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human TSC2 (CL488-68380, Clone:1A10B2)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated TSC2 Monoclonal antibody

TSC2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68380

产品说明书

CloneNo. 1A10B2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

Tuberin, tuberous sclerosis 2, Tuberin/TSC2, TSC4, 1A10B2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy TSC2 Ready-To-Use IHC Kit. TSC2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68380-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68380 targets TSC2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tuberous sclerosis 2
别名 Tuberin, tuberous sclerosis 2, Tuberin/TSC2, TSC4, 1A10B2
计算分子量 1807 aa, 201 kDa
观测分子量 200 kDa
GenBank蛋白编号BC150300
基因名称 TSC2
Gene ID (NCBI) 7249
RRIDAB_3672999
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP49815
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TSC2 antibody CL488-68380Download protocol
FC protocol for CL Plus 488 TSC2 antibody CL488-68380Download protocol
Standard Protocols
Click here to view our Standard Protocols