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  • Featured Product
  • KD/KO Validated

Tuberin/TSC2 Monoclonal antibody, PBS Only

Tuberin/TSC2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A10B2

Cat no : 68380-1-PBS

Print datasheet

Synonyms

TSC2, Tuberin, tuberous sclerosis 2, TSC4, 1A10B2

验证数据展示

  • WB analysis using 68380-1-Ig (same clone as 68380-1-PBS)

    WB analysis using 68380-1-Ig (same clone as 68380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68380-1-Ig (same clone as 68380-1-PBS)

    WB analysis of HeLa using 68380-1-Ig (same clone as 68380-1-PBS)

    WB result of Tuberin/TSC2 antibody (68380-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Tuberin/TSC2 transfected HeLa cells. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 68380-1-Ig (same clone as 68380-1-PBS)

    IF Staining of HEK-293 using 68380-1-Ig (same clone as 68380-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TSC2 antibody (68380-1-Ig, Clone: 1A10B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 68380-1-Ig (same clone as 68380-1-PBS)

    WB analysis of MCF-7 using 68380-1-Ig (same clone as 68380-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

  • WB analysis of PC-3 using 68380-1-Ig (same clone as 68380-1-PBS)

    WB analysis of PC-3 using 68380-1-Ig (same clone as 68380-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 68380-1-Ig (same clone as 68380-1-PBS)

    WB analysis of NIH/3T3 using 68380-1-Ig (same clone as 68380-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 68380-1-Ig (same clone as 68380-1-PBS)

    WB analysis of HSC-T6 using 68380-1-Ig (same clone as 68380-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 68380-1-Ig (same clone as 68380-1-PBS)

    IHC staining of rat brain using 68380-1-Ig (same clone as 68380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TSC2 antibody included
  • IHC staining of rat brain using 68380-1-Ig (same clone as 68380-1-PBS)

    IHC staining of rat brain using 68380-1-Ig (same clone as 68380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TSC2 antibody included
  • IHC staining of human lung cancer using 68380-1-Ig (same clone as 68380-1-PBS)

    IHC staining of human lung cancer using 68380-1-Ig (same clone as 68380-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TSC2 antibody included
Planning an IHC experiment? We recommend our IHCeasy TSC2 Ready-To-Use IHC Kit. TSC2 primary antibody included.

产品信息

68380-1-PBS targets Tuberin/TSC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tuberous sclerosis 2
Synonyms TSC2, Tuberin, tuberous sclerosis 2, TSC4, 1A10B2
Calculated Molecular Weight 1807 aa, 201 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberBC150300
Gene Symbol TSC2
Gene ID (NCBI) 7249
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49815
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling.