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  • Featured Product
  • KD/KO Validated

TSC2 Monoclonal antibody, PBS Only

TSC2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A10B2

Cat no : 68380-1-PBS

Print datasheet

Synonyms

Tuberin, tuberous sclerosis 2, Tuberin/TSC2, TSC4, 1A10B2

验证数据展示

  • WB analysis using 68380-1-Ig (same clone as 68380-1-PBS)

    WB analysis using 68380-1-Ig (same clone as 68380-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68380-1-Ig (same clone as 68380-1-PBS)

    WB analysis of HeLa using 68380-1-Ig (same clone as 68380-1-PBS)

    WB result of Tuberin/TSC2 antibody (68380-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Tuberin/TSC2 transfected HeLa cells. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 68380-1-Ig (same clone as 68380-1-PBS)

    IF Staining of HEK-293 using 68380-1-Ig (same clone as 68380-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TSC2 antibody (68380-1-Ig, Clone: 1A10B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 68380-1-Ig (same clone as 68380-1-PBS)

    WB analysis of MCF-7 using 68380-1-Ig (same clone as 68380-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

  • WB analysis of PC-3 using 68380-1-Ig (same clone as 68380-1-PBS)

    WB analysis of PC-3 using 68380-1-Ig (same clone as 68380-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 68380-1-Ig (same clone as 68380-1-PBS)

    WB analysis of NIH/3T3 using 68380-1-Ig (same clone as 68380-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 68380-1-Ig (same clone as 68380-1-PBS)

    WB analysis of HSC-T6 using 68380-1-Ig (same clone as 68380-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

产品信息

68380-1-PBS targets TSC2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tuberous sclerosis 2
Synonyms Tuberin, tuberous sclerosis 2, Tuberin/TSC2, TSC4, 1A10B2
Calculated Molecular Weight 1807 aa, 201 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberBC150300
Gene Symbol TSC2
Gene ID (NCBI) 7249
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49815
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling.