TSG101 Monoclonal antibody
TSG101 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat and More (3)
Applications
FC, IF, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2B7G8
验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HSC-T6 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, C6 cells, NIH/3T3 cells |
Positive IHC detected in | human lung cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, human colon cancer tissue |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:1000-1:4000 |
Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 20 publications below |
产品信息
67381-1-Ig targets TSG101 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | FC, IF, IHC, WB, ELISA |
Cited Applications | WB |
Tested Reactivity | Human, Mouse, Rat |
Cited Reactivity | human, mouse, rat |
Immunogen | TSG101 fusion protein Ag28569 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | tumor susceptibility gene 101 |
Synonyms | Cell and organelle markers, ESCRT I complex subunit TSG101, Exosome marker, TSG10, TSG101, tumor susceptibility gene 101, VPS23 |
Calculated Molecular Weight | 44 kDa |
Observed Molecular Weight | 43-46 kDa |
GenBank Accession Number | BC002487 |
Gene Symbol | TSG101 |
Gene ID (NCBI) | 7251 |
RRID | AB_2882628 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q99816 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for TSG101 antibody 67381-1-Ig | Download protocol |
IHC protocol for TSG101 antibody 67381-1-Ig | Download protocol |
IF protocol for TSG101 antibody 67381-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Bioact Mater Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration. | ||
Oxid Med Cell Longev Adipose-Derived Circulating Exosomes Promote Protection of the Pulmonary Endothelial Barrier by Inhibiting EndMT and Oxidative Stress through Down-Regulation of the TGF-β Pathway: A Potential Explanation for the Obesity Paradox in ARDS. | ||
J Nanobiotechnology Extracellular vesicles derived from hypoxia-preconditioned olfactory mucosa mesenchymal stem cells enhance angiogenesis via miR-612 | ||
Aging (Albany NY) Endothelial cell-derived exosomal circHIPK3 promotes the proliferation of vascular smooth muscle cells induced by high glucose via the miR-106a-5p/Foxo1/Vcam1 pathway. | ||
Int J Biol Macromol Exosomal miRNA-181a-5p from the cells of the hair follicle dermal papilla promotes the hair follicle growth and development via the Wnt/β-catenin signaling pathway. | ||
Front Microbiol Proteomics Analysis of Exosomes From Patients With Active Tuberculosis Reveals Infection Profiles and Potential Biomarkers. |