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验证数据展示

  • FC experiment of HeLa using CL647-67381

    FC experiment of HeLa using CL647-67381

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human TSG101 (CL647-67381, Clone:2B7G8) (red), or 0.2 ug isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 647-conjugated TSG101 Monoclonal antibody

TSG101 Monoclonal Antibody for FC (Intra)
Cat No. CL647-67381

产品说明书

CloneNo. 2B7G8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

VPS 23, TSG10, TSG 101, TSG 10, ESCRT-I complex subunit TSG101

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TSG101 Ready-To-Use IHC Kit. TSG101 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67381-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67381 targets TSG101 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 TSG101 fusion protein Ag28569 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 tumor susceptibility gene 101
别名 VPS 23, TSG10, TSG 101, TSG 10, ESCRT-I complex subunit TSG101
计算分子量 44 kDa
观测分子量 43-46 kDa
GenBank蛋白编号BC002487
基因名称 TSG101
Gene ID (NCBI) 7251
RRIDAB_2935078
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ99816
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 TSG101 antibody CL647-67381Download protocol
Standard Protocols
Click here to view our Standard Protocols