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CoraLite® Plus 488-conjugated TSG101 Monoclonal antibody

TSG101 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B7G8

Cat no : CL488-67381

Print datasheet

Synonyms

VPS 23, TSG10, TSG 101, TSG 10, ESCRT-I complex subunit TSG101

验证数据展示

  • IF Staining of human colon cancer using CL488-67381

    IF Staining of human colon cancer using CL488-67381

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488-conjugated TSG101 antibody (CL488-67381, Clone: 2B7G8 ) at dilution of 1:200.

  • IF Staining of human colon cancer using CL488-67381

    IF Staining of human colon cancer using CL488-67381

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488-conjugated TSG101 antibody (CL488-67381, Clone: 2B7G8 ) at dilution of 1:200.

  • FC experiment of THP-1 using CL488-67381

    FC experiment of THP-1 using CL488-67381

    1X10^6 THP-1 cells were stained with 0.2 ug CoraLite® Plus 488 Anti-Human TSG101 (CL488-67381, Clone: 2B7G8) (red), or 0.2 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF-P detected inhuman colon cancer tissue
Positive FC (Intra) detected inTHP-1 cells
Planning an IHC experiment? We recommend our IHCeasy TSG101 Ready-To-Use IHC Kit. TSG101 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67381-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67381 targets TSG101 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen TSG101 fusion protein Ag28569 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tumor susceptibility gene 101
Synonyms VPS 23, TSG10, TSG 101, TSG 10, ESCRT-I complex subunit TSG101
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 43-46 kDa
GenBank Accession NumberBC002487
Gene Symbol TSG101
Gene ID (NCBI) 7251
RRIDAB_2919462
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99816
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TSG101 antibody CL488-67381Download protocol
Standard Protocols
Click here to view our Standard Protocols