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验证数据展示

  • WB analysis of A2780 using 13722-1-AP (same clone as 13722-1-PBS)

    WB analysis of A2780 using 13722-1-AP (same clone as 13722-1-PBS)

    A2780 cells were subjected to SDS PAGE followed by western blot with 13722-1-AP (TTF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13722-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 13722-1-AP (same clone as 13722-1-PBS)

    IHC staining of human ovary tumor using 13722-1-AP (same clone as 13722-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13722-1-AP (TTF2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13722-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TTF2 antibody included
  • IP experiment of A2780 using 13722-1-AP (same clone as 13722-1-PBS)

    IP experiment of A2780 using 13722-1-AP (same clone as 13722-1-PBS)

    IP result of anti-TTF2 (IP:13722-1-AP, 4ug; Detection:13722-1-AP 1:500) with A2780 cells lysate 840ug. This data was developed using the same antibody clone with 13722-1-PBS in a different storage buffer formulation.

  • WB analysis using 13722-1-AP (same clone as 13722-1-PBS)

    WB analysis using 13722-1-AP (same clone as 13722-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13722-1-AP (TTF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13722-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 13722-1-AP (same clone as 13722-1-PBS)

    IF Staining of HeLa using 13722-1-AP (same clone as 13722-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TTF2 antibody (13722-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (black). This data was developed using the same antibody clone with 13722-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 13722-1-AP (same clone as 13722-1-PBS)

    IF Staining of HeLa using 13722-1-AP (same clone as 13722-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TTF2 antibody (13722-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13722-1-PBS in a different storage buffer formulation.

TTF2 Polyclonal antibody, PBS Only

TTF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13722-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TTF2 Ready-To-Use IHC Kit. TTF2 primary antibody included.

产品信息

13722-1-PBS targets TTF2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TTF2 fusion protein Ag4671 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transcription termination factor, RNA polymerase II
别名
计算分子量 1162 aa, 129 kDa
观测分子量 130 kDa
GenBank蛋白编号BC030058
基因名称 TTF2
Gene ID (NCBI) 8458
RRIDAB_2262865
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UNY4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transcription elongation is an key target for control of eukaryotic gene expression. After initiating from a promoter, the RNA polymerase II elongation complex is subjected to a block to elongation by the action of components of N-TEF (negative transcription elongation factor) [PMID: 9748214]. Transcription termination factor 2 (TTF2) is a DsDNA-dependent ATPase that acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. It also contribute to mitotic transcription repression, and involved in pre-mRNA splicing[PMID:15125840, 12927788].