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验证数据展示

  • IF Staining of HepG2 using CL488-66544

    IF Staining of HepG2 using CL488-66544

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated TWIST2 antibody (CL488-66544, Clone: 1B12D11 ) at dilution of 1:100.

CoraLite® Plus 488-conjugated TWIST2 Monoclonal antibody

TWIST2 Monoclonal Antibody for IF/ICC
Cat No. CL488-66544

产品说明书

CloneNo. 1B12D11

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat, mouse

应用

IF/ICC

别名

bHLHa39, Dermis expressed protein 1, Dermo 1, DERMO1, twist homolog 2 (Drosophila), Twist related protein 2, TWIST2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TWIST2 Ready-To-Use IHC Kit. TWIST2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66544-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66544 targets TWIST2 in IF/ICC applications and shows reactivity with Human, rat, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, rat, mouse
免疫原 TWIST2 fusion protein Ag2348 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 twist homolog 2 (Drosophila)
别名 bHLHa39, Dermis expressed protein 1, Dermo 1, DERMO1, twist homolog 2 (Drosophila), Twist related protein 2, TWIST2
计算分子量 160 aa, 18 kDa
观测分子量 22 kDa
GenBank蛋白编号BC033168
基因名称 TWIST2
Gene ID (NCBI) 117581
RRIDAB_2919341
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8WVJ9
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TWIST2, also named as BHLHA39 and DERMO1,is a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. TWIST2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors. It is also implicated in tumorigenesis, invasion and metastasis. TWIST2 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TWIST2 antibody CL488-66544Download protocol
Standard Protocols
Click here to view our Standard Protocols