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验证数据展示

  • FC experiment of HeLa using CL594-66544

    FC experiment of HeLa using CL594-66544

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TWIST2 (CL594-66544, Clone:1B12D11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL594-66544

    IF Staining of HepG2 using CL594-66544

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 TWIST2 antibody (CL594-66544, Clone: 1B12D11 ) at dilution of 1:200.

CoraLite®594-conjugated TWIST2 Monoclonal antibody

TWIST2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66544

产品说明书

CloneNo. 1B12D11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Dermo-1, DERMO1, Dermis-expressed protein 1, Class A basic helix-loop-helix protein 39, bHLHa39

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TWIST2 Ready-To-Use IHC Kit. TWIST2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66544-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66544 targets TWIST2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 TWIST2 fusion protein Ag2348 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 twist homolog 2 (Drosophila)
别名 Dermo-1, DERMO1, Dermis-expressed protein 1, Class A basic helix-loop-helix protein 39, bHLHa39
计算分子量 160 aa, 18 kDa
观测分子量 22 kDa
GenBank蛋白编号BC033168
基因名称 TWIST2
Gene ID (NCBI) 117581
RRIDAB_3673504
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8WVJ9
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TWIST2, also named as BHLHA39 and DERMO1,is a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. TWIST2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors. It is also implicated in tumorigenesis, invasion and metastasis. TWIST2 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).

实验方案

Product Specific Protocols
IF protocol for CL594 TWIST2 antibody CL594-66544Download protocol
FC protocol for CL594 TWIST2 antibody CL594-66544Download protocol
Standard Protocols
Click here to view our Standard Protocols