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CoraLite®594-conjugated TWIST2 Monoclonal antibody

TWIST2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B12D11

Cat no : CL594-66544

Print datasheet

Synonyms

Dermo-1, DERMO1, Dermis-expressed protein 1, Class A basic helix-loop-helix protein 39, bHLHa39

验证数据展示

  • FC experiment of HeLa using CL594-66544

    FC experiment of HeLa using CL594-66544

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TWIST2 (CL594-66544, Clone:1B12D11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL594-66544

    IF Staining of HepG2 using CL594-66544

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 TWIST2 antibody (CL594-66544, Clone: 1B12D11 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TWIST2 Ready-To-Use IHC Kit. TWIST2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66544-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66544 targets TWIST2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen TWIST2 fusion protein Ag2348 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name twist homolog 2 (Drosophila)
Synonyms Dermo-1, DERMO1, Dermis-expressed protein 1, Class A basic helix-loop-helix protein 39, bHLHa39
Calculated Molecular Weight 160 aa, 18 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC033168
Gene Symbol TWIST2
Gene ID (NCBI) 117581
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8WVJ9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TWIST2, also named as BHLHA39 and DERMO1,is a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. TWIST2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis, possibly by changing the internal signal transduction response of osteoblasts to external growth factors. It is also implicated in tumorigenesis, invasion and metastasis. TWIST2 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).

实验方案

Product Specific Protocols
IF protocol for CL594 TWIST2 antibody CL594-66544Download protocol
FC protocol for CL594 TWIST2 antibody CL594-66544Download protocol
Standard Protocols
Click here to view our Standard Protocols