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Talin-1 Monoclonal antibody, PBS Only

Talin-1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E12D10

Cat No : 67992-1-PBS

Print datasheet

Synonyms

ILWEQ, KIAA1027, talin 1, TLN, TLN1

验证数据展示

  • WB analysis using 67992-1-Ig (same clone as 67992-1-PBS)

    WB analysis using 67992-1-Ig (same clone as 67992-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67992-1-Ig (Talin-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67992-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67992-1-Ig (same clone as 67992-1-PBS)

    IHC staining of human colon cancer using 67992-1-Ig (same clone as 67992-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67992-1-Ig (Talin-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67992-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67992-1-Ig (same clone as 67992-1-PBS)

    IHC staining of human colon cancer using 67992-1-Ig (same clone as 67992-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67992-1-Ig (Talin-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67992-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67992-1-Ig (same clone as 67992-1-PBS)

    FC experiment of HeLa using 67992-1-Ig (same clone as 67992-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Talin-1 (67992-1-Ig, Clone:2E12D10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67992-1-PBS in a different storage buffer formulation.

67992-1-Ig

产品信息

67992-1-PBS targets Talin-1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen Talin-1 fusion protein Ag5937 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name talin 1
Synonyms ILWEQ, KIAA1027, talin 1, TLN, TLN1
Calculated Molecular Weight 2541 aa, 270 kDa
Observed Molecular Weight 270 kDa
GenBank Accession NumberBC042923
Gene Symbol TLN1
Gene ID (NCBI) 7094
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y490
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).