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  • Featured Product
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Talin-1 Monoclonal antibody

Talin-1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2E12D10

Cat no : 67992-1-Ig

Print datasheet

Synonyms

TLN1, TLN, talin 1, KIAA1027, ILWEQ

验证数据展示

  • WB analysis using 67992-1-Ig

    WB analysis using 67992-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67992-1-Ig (Talin-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67992-1-Ig

    WB analysis of HeLa using 67992-1-Ig

    WB result of Talin-1 antibody (67992-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Talin-1 transfected HeLa cells.

  • IHC staining of human colon cancer using 67992-1-Ig

    IHC staining of human colon cancer using 67992-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67992-1-Ig (Talin-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 67992-1-Ig

    IHC staining of human colon cancer using 67992-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67992-1-Ig (Talin-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 67992-1-Ig

    FC experiment of HeLa using 67992-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Talin-1 (67992-1-Ig, Clone:2E12D10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A549 using 67992-1-Ig

    IF Staining of A549 using 67992-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (67992-1-Ig, Clone: 2E12D10 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

经过测试的应用

Positive WB detected inHeLa cells, human peripheral blood platelets, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 750 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67992-1-Ig targets Talin-1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Talin-1 fusion protein Ag5937 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name talin 1
Synonyms TLN1, TLN, talin 1, KIAA1027, ILWEQ
Calculated Molecular Weight 2541 aa, 270 kDa
Observed Molecular Weight 270 kDa
GenBank Accession NumberBC042923
Gene Symbol Talin-1
Gene ID (NCBI) 7094
RRIDAB_2918741
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y490
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

实验方案

Product Specific Protocols
WB protocol for Talin-1 antibody 67992-1-IgDownload protocol
IHC protocol for Talin-1 antibody 67992-1-IgDownload protocol
IF protocol for Talin-1 antibody 67992-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols