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验证数据展示

  • FC experiment of HeLa using CL750-67992

    FC experiment of HeLa using CL750-67992

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 750 Anti-Human Talin-1 (CL750-67992, Clone:2E12D10) (red), or 0.8 ug CoraLite® Plus 750 Mouse IgG1 Isotype Control (MOPC-21) (CL750-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 750-conjugated Talin-1 Monoclonal antibody

Talin-1 Monoclonal Antibody for FC (Intra)
Cat No. CL750-67992
CloneNo. 2E12D10

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Synonyms

ILWEQ, KIAA1027, talin 1, TLN, TLN1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 750
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67992-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-67992 targets Talin-1 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human
Immunogen Talin-1 fusion protein Ag5937 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name talin 1
Synonyms ILWEQ, KIAA1027, talin 1, TLN, TLN1
Calculated Molecular Weight 2541 aa, 270 kDa
Observed Molecular Weight 270 kDa
GenBank Accession NumberBC042923
Gene Symbol Talin-1
Gene ID (NCBI) 7094
RRIDAB_3673720
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y490
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

实验方案

Product Specific Protocols
FC protocol for CL Plus 750 Talin-1 antibody CL750-67992Download protocol
Standard Protocols
Click here to view our Standard Protocols