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CoraLite® Plus 750-conjugated Talin-1 Monoclonal antibody

Talin-1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2E12D10

Cat No : CL750-67992

Print datasheet

Synonyms

ILWEQ, KIAA1027, talin 1, TLN, TLN1

验证数据展示

  • FC experiment of HeLa using CL750-67992

    FC experiment of HeLa using CL750-67992

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 750 Anti-Human Talin-1 (CL750-67992, Clone:2E12D10) (red), or 0.8 ug CoraLite® Plus 750 Mouse IgG1 Isotype Control (MOPC-21) (CL750-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67992-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-67992 targets Talin-1 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human
Immunogen Talin-1 fusion protein Ag5937 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name talin 1
Synonyms ILWEQ, KIAA1027, talin 1, TLN, TLN1
Calculated Molecular Weight 2541 aa, 270 kDa
Observed Molecular Weight 270 kDa
GenBank Accession NumberBC042923
Gene Symbol TLN1
Gene ID (NCBI) 7094
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y490
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

实验方案

Product Specific Protocols
FC protocol for CL Plus 750 Talin-1 antibody CL750-67992Download protocol
Standard Protocols
Click here to view our Standard Protocols