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CoraLite® Plus 488-conjugated Talin-1 Monoclonal antibody
Talin-1 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2E12D10
验证数据展示
经过测试的应用
Positive FC (Intra) detected in | HeLa cells |
Positive FC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 67992-1-Ig
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67992 targets Talin-1 in FC (Intra) applications and shows reactivity with Human samples.
Tested Applications | FC (Intra) Application Description |
Tested Reactivity | Human |
Immunogen | Talin-1 fusion protein Ag5937 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | talin 1 |
Synonyms | ILWEQ, KIAA1027, talin 1, TLN, TLN1 |
Calculated Molecular Weight | 2541 aa, 270 kDa |
Observed Molecular Weight | 270 kDa |
GenBank Accession Number | BC042923 |
Gene Symbol | Talin-1 |
Gene ID (NCBI) | 7094 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q9Y490 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 Talin-1 antibody CL488-67992 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |