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CoraLite® Plus 488-conjugated Talin-1 Recombinant antibody
Talin-1 Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
240294G5
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A549 cells |
For other applications, we recommend the unconjugated version of this antibody, 82856-18-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of CL488-82856-18 is Talin-1 Fusion Protein expressed in E. coli.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | Talin-1 fusion protein Ag5367 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | talin 1 |
| Synonyms | TLN1, TLN, talin 1, KIAA1027, ILWEQ |
| Calculated Molecular Weight | 2541 aa, 270 kDa |
| Observed Molecular Weight | ~270 kDa |
| GenBank Accession Number | BC042923 |
| Gene Symbol | Talin-1 |
| Gene ID (NCBI) | 7094 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y490 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Talin-1 antibody CL488-82856-18 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |