验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A549 cells |
For other applications, we recommend the unconjugated version of this antibody, 82856-18-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-82856-18 targets Talin-1 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | Talin-1 fusion protein Ag5367 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | talin 1 |
| 别名 | TLN, talin1, talin 1, KIAA1027, ILWEQ |
| 计算分子量 | 2541 aa, 270 kDa |
| GenBank蛋白编号 | BC042923 |
| 基因名称 | Talin-1 |
| Gene ID (NCBI) | 7094 |
| RRID | AB_3673531 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9Y490 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 Talin-1 antibody CL594-82856-18 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |