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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 750-conjugated Talin-1 Polyclonal antibody

Talin-1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

Cat no : CL750-14168

Print datasheet

Synonyms

TLN1, TLN, talin 1, KIAA1027, ILWEQ

验证数据展示

  • FC experiment of HeLa using CL750-14168

    FC experiment of HeLa using CL750-14168

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 750-conjugated Talin-1 Polyclonal antibody (CL750-14168) (red), or 0.8 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A549 using CL750-14168

    IF Staining of A549 using CL750-14168

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 750 Talin-1 antibody (CL750-14168) at dilution of 1:200, CL594-phalloidin (red).

经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 14168-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-14168 targets Talin-1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Talin-1 fusion protein Ag5367 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name talin 1
Synonyms TLN1, TLN, talin 1, KIAA1027, ILWEQ
Calculated Molecular Weight 2541 aa, 270 kDa
Observed Molecular Weight 250-270 kDa
GenBank Accession NumberBC042923
Gene Symbol Talin-1
Gene ID (NCBI) 7094
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y490
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

实验方案

Product Specific Protocols
IF protocol for CL Plus 750 Talin-1 antibody CL750-14168Download protocol
Standard Protocols
Click here to view our Standard Protocols