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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Talin-1 Polyclonal antibody

Talin-1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-14168

Print datasheet

Synonyms

TLN1, TLN, talin 1, KIAA1027, ILWEQ

验证数据展示

  • IF Staining of A549 using CL488-14168

    IF Staining of A549 using CL488-14168

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 Talin-1 antibody (CL488-14168) at dilution of 1:200.

  • FC experiment of HeLa using CL488-14168

    FC experiment of HeLa using CL488-14168

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Talin-1 (CL488-14168) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 14168-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14168 targets Talin-1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Talin-1 fusion protein Ag5367 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name talin 1
Synonyms TLN1, TLN, talin 1, KIAA1027, ILWEQ
Calculated Molecular Weight 2541 aa, 270 kDa
Observed Molecular Weight 270 kDa, 230 kDa
GenBank Accession NumberBC042923
Gene Symbol TLN1
Gene ID (NCBI) 7094
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y490
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Talin-1 antibody CL488-14168Download protocol
Standard Protocols
Click here to view our Standard Protocols