验证数据展示
经过测试的应用
| Positive FC detected in | LPS treated human PBMCs |
For other applications, we recommend the unconjugated version of this antibody, 98104-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-98104 targets Tissue Factor/CD142 in FC applications and shows reactivity with human samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | human |
| Immunogen | Recombinant protein 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | coagulation factor III (thromboplastin, tissue factor) |
| Synonyms | F3, Coagulation factor III, CD142, Tissue factor, TFA |
| Calculated Molecular Weight | 33 kDa |
| GenBank Accession Number | NM_001993.5 |
| Gene Symbol | F3 |
| Gene ID (NCBI) | 2152 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P13726-1 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named CD142, coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 Tissue Factor/CD142 antibody CL647-98104 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |