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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83628-1-RR (same clone as 83628-1-PBS)

    WB analysis using 83628-1-RR (same clone as 83628-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83628-1-RR (UBC12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83628-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 83628-1-RR (same clone as 83628-1-PBS)

    FC experiment of HEK-293 using 83628-1-RR (same clone as 83628-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug UBC12 Recombinant antibody (83628-1-RR, Clone:240477H5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83628-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83628-1-RR

    Affinity and Kinetic Characterization of 83628-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83628-1-RR against Human UBC12 were performed. The affinity constant is 2.45 nM.

UBC12 Recombinant antibody, PBS Only

UBC12 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83628-1-PBS

产品说明书

CloneNo. 240477H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

UBE2M, NEDD8 protein ligase, NEDD8 conjugating enzyme Ubc12, NEDD8 carrier protein, hUbc12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83628-1-PBS targets UBC12 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 UBC12 fusion protein Ag5984 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
别名 UBE2M, NEDD8 protein ligase, NEDD8 conjugating enzyme Ubc12, NEDD8 carrier protein, hUbc12
计算分子量 21 kDa
观测分子量 17-20 kDa
GenBank蛋白编号BC058924
基因名称 UBC12
Gene ID (NCBI) 9040
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP61081
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

UBE2M (Ubiquitin-conjugating enzyme E2 M), also called UBC12, accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction between UBE2M and the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation (PMID: 10207026; 15361859).