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UBC12 Recombinant antibody

UBC12 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240477H5

Cat no : 83628-1-RR

Print datasheet

Synonyms

UBE2M, NEDD8 protein ligase, NEDD8 conjugating enzyme Ubc12, NEDD8 carrier protein, hUbc12

验证数据展示

  • WB analysis using 83628-1-RR

    WB analysis using 83628-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83628-1-RR (UBC12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293 using 83628-1-RR

    FC experiment of HEK-293 using 83628-1-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug UBC12 Recombinant antibody (83628-1-RR, Clone:240477H5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83628-1-RR

    Affinity and Kinetic Characterization of 83628-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83628-1-RR against Human UBC12 were performed. The affinity constant is 2.45 nM.

经过测试的应用

Positive WB detected inHEK-293 cells, L02 cells, SMMC-7721 cells, A549 cells, HT-1080 cells
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83628-1-RR targets UBC12 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen UBC12 fusion protein Ag5984 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
Synonyms UBE2M, NEDD8 protein ligase, NEDD8 conjugating enzyme Ubc12, NEDD8 carrier protein, hUbc12
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 17-20 kDa
GenBank Accession NumberBC058924
Gene Symbol UBC12
Gene ID (NCBI) 9040
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP61081
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBE2M (Ubiquitin-conjugating enzyme E2 M), also called UBC12, accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction between UBE2M and the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation (PMID: 10207026; 15361859).

实验方案

Product Specific Protocols
WB protocol for UBC12 antibody 83628-1-RRDownload protocol
FC protocol for UBC12 antibody 83628-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols