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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated UCH-L1/PGP9.5 Monoclonal antibody

UCH-L1/PGP9.5 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C9E11

Cat no : CL488-66230

Print datasheet

Synonyms

PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5

验证数据展示

  • IF Staining of mouse brain using CL488-66230

    IF Staining of mouse brain using CL488-66230

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 UCHL1/PGP9.5 antibody (CL488-66230, Clone: 1C9E11 ) at dilution of 1:200.

  • IF Staining of SH-SY5Y using CL488-66230

    IF Staining of SH-SY5Y using CL488-66230

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 UCHL1/PGP9.5 antibody (CL488-66230, Clone: 1C9E11 ) at dilution of 1:200.

  • FC experiment of Y79 using CL488-66230

    FC experiment of Y79 using CL488-66230

    1X10^6 Y79 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human UCHL1/PGP9.5 (CL488-66230, Clone:1C9E11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inY79 cells
Planning an IHC experiment? We recommend our IHCeasy UCHL1 Ready-To-Use IHC Kit. UCHL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66230-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66230 targets UCH-L1/PGP9.5 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC, IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen UCH-L1/PGP9.5 fusion protein Ag6547 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Synonyms PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC000332
Gene Symbol UCHL1
Gene ID (NCBI) 7345
RRIDAB_2883290
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP09936
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 UCH-L1/PGP9.5 antibody CL488-66230Download protocol
Standard Protocols
Click here to view our Standard Protocols