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验证数据展示

  • IF Staining of mouse brain using CL488-66230

    IF Staining of mouse brain using CL488-66230

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 UCHL1/PGP9.5 antibody (CL488-66230, Clone: 1C9E11 ) at dilution of 1:200.

  • IF Staining of SH-SY5Y using CL488-66230

    IF Staining of SH-SY5Y using CL488-66230

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 UCHL1/PGP9.5 antibody (CL488-66230, Clone: 1C9E11 ) at dilution of 1:200.

  • FC experiment of Y79 using CL488-66230

    FC experiment of Y79 using CL488-66230

    1X10^6 Y79 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human UCHL1/PGP9.5 (CL488-66230, Clone:1C9E11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated UCH-L1/PGP9.5 Monoclonal antibody

UCH-L1/PGP9.5 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)
Cat No. CL488-66230

产品说明书

CloneNo. 1C9E11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF/ICC, IF-P, FC (Intra)

别名

PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inY79 cells
Planning an IHC experiment? We recommend our IHCeasy UCHL1 Ready-To-Use IHC Kit. UCHL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66230-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66230 targets UCH-L1/PGP9.5 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 UCH-L1/PGP9.5 fusion protein Ag6547 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
别名 PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5
计算分子量 25 kDa
观测分子量 27 kDa
GenBank蛋白编号BC000332
基因名称 UCHL1
Gene ID (NCBI) 7345
RRIDAB_2883290
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP09936
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 UCH-L1/PGP9.5 antibody CL488-66230Download protocol
Standard Protocols
Click here to view our Standard Protocols