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验证数据展示

  • IF Staining of SH-SY5Y using CL647-66230

    IF Staining of SH-SY5Y using CL647-66230

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CoraLite® Plus 647 UCHL1/PGP9.5 antibody (CL647-66230, Clone: 1C9E11 ) at dilution of 1:200.

  • FC experiment of SH-SY5Y using CL647-66230

    FC experiment of SH-SY5Y using CL647-66230

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 647-conjugated UCHL1/PGP9.5 Monoclonal antibody (CL647-66230, Clone:1C9E11)(red), or 0.8 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (MOPC-21) (CL647-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated UCH-L1/PGP9.5 Monoclonal antibody

UCH-L1/PGP9.5 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-66230

产品说明书

CloneNo. 1C9E11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF/ICC, FC (Intra)

别名

PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy UCHL1 Ready-To-Use IHC Kit. UCHL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66230-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66230 targets UCH-L1/PGP9.5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原 UCH-L1/PGP9.5 fusion protein Ag6547 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
别名 PGP9.5, UCHL1, UCHL1/PGP9.5, PGP 9.5, PARK5
计算分子量 25 kDa
观测分子量 27 kDa
GenBank蛋白编号BC000332
基因名称 UCHL1
Gene ID (NCBI) 7345
RRIDAB_2935046
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP09936
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ubiquitin C-terminal hydrolase L1 (UCHL1) was originally identified as a neuronal protein that accounts for nearly 2% of total brain proteins. UCHL1 activity protects neurons from hypoxic injury, and binding of stroke-induced reactive lipid species to the cysteine 152 (C152) of UCHL1 unfolds the protein and disrupts its function. Reduced hydrolytic activity of mutant UCHL1 is implicated in the pathophysiologic process of Parkinson's and Alzheimer's disease due to abnormal neurotoxic protein aggregation. (PMID: 31356902, PMID: 30760601)

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 UCH-L1/PGP9.5 antibody CL647-66230Download protocol
Standard Protocols
Click here to view our Standard Protocols