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CoraLite® Plus 647-conjugated UCHL1/PGP9.5 Monoclonal antibody
UCHL1/PGP9.5 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, pig, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1C9E11
验证数据展示
经过测试的应用
Positive IF detected in | SH-SY5Y cells |
Planning an IHC experiment? We recommend our IHCeasy UCHL1 Ready-To-Use IHC Kit. UCHL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66230-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-66230 targets UCHL1/PGP9.5 in IF applications and shows reactivity with human, mouse, pig, rat samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human, mouse, pig, rat |
Immunogen | UCHL1/PGP9.5 fusion protein Ag6547 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) |
Synonyms | Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1, Uch L1, UCHL1 |
Calculated Molecular Weight | 25 kDa |
Observed Molecular Weight | 27 kDa |
GenBank Accession Number | BC000332 |
Gene Symbol | UCHL1 |
Gene ID (NCBI) | 7345 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P09936 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ubiquitin C-terminal hydrolase L1 (UCHL1) was originally identified as a neuronal protein that accounts for nearly 2% of total brain proteins. UCHL1 activity protects neurons from hypoxic injury, and binding of stroke-induced reactive lipid species to the cysteine 152 (C152) of UCHL1 unfolds the protein and disrupts its function. Reduced hydrolytic activity of mutant UCHL1 is implicated in the pathophysiologic process of Parkinson's and Alzheimer's disease due to abnormal neurotoxic protein aggregation. (PMID: 31356902, PMID: 30760601)
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 647 UCHL1/PGP9.5 antibody CL647-66230 | Download protocol |
FC protocol for CL Plus 647 UCHL1/PGP9.5 antibody CL647-66230 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |