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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated UTP14A Polyclonal antibody

UTP14A Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11474

Print datasheet

Synonyms

U3 small nucleolar RNA-associated protein 14 homolog A, SDCCAG16, NY CO 16, KIAA0266, Antigen NY-CO-16

验证数据展示

  • IF Staining of HepG2 using CL488-11474

    IF Staining of HepG2 using CL488-11474

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 UTP14A antibody (CL488-11474) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HepG2 using CL488-11474

    FC experiment of HepG2 using CL488-11474

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Utp14a Polyclonal Antibody (CL488-11474)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 11474-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11474 targets UTP14A in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen UTP14A fusion protein Ag2027 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name UTP14, U3 small nucleolar ribonucleoprotein, homolog A (yeast)
Synonyms U3 small nucleolar RNA-associated protein 14 homolog A, SDCCAG16, NY CO 16, KIAA0266, Antigen NY-CO-16
Calculated Molecular Weight 771 aa, 88 kDa
Observed Molecular Weight 88 kDa
GenBank Accession NumberBC014987
Gene Symbol UTP14A
Gene ID (NCBI) 10813
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BVJ6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 UTP14A antibody CL488-11474Download protocol
FC protocol for CL Plus 488 UTP14A antibody CL488-11474Download protocol
Standard Protocols
Click here to view our Standard Protocols