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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 30599-1-AP (same clone as 30599-1-PBS)

    WB analysis using 30599-1-AP (same clone as 30599-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30599-1-AP (VCAN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

  • IHC staining of human endometrial cancer using 30599-1-AP (same clone as 30599-1-PBS)

    IHC staining of human endometrial cancer using 30599-1-AP (same clone as 30599-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30599-1-AP (VCAN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

  • IHC staining of human endometrial cancer using 30599-1-AP (same clone as 30599-1-PBS)

    IHC staining of human endometrial cancer using 30599-1-AP (same clone as 30599-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30599-1-AP (VCAN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 30599-1-AP (same clone as 30599-1-PBS)

    IHC staining of human stomach cancer using 30599-1-AP (same clone as 30599-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30599-1-AP (VCAN antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 30599-1-AP (same clone as 30599-1-PBS)

    IHC staining of human stomach cancer using 30599-1-AP (same clone as 30599-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30599-1-AP (VCAN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 30599-1-AP (same clone as 30599-1-PBS)

    IF Staining of NIH/3T3 using 30599-1-AP (same clone as 30599-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using VCAN antibody (30599-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 30599-1-AP (same clone as 30599-1-PBS)

    IF Staining of NIH/3T3 using 30599-1-AP (same clone as 30599-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using VCAN antibody (30599-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 30599-1-PBS in a different storage buffer formulation.

VCAN Polyclonal antibody, PBS Only

VCAN Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30599-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PG M, Large fibroblast proteoglycan, Glial hyaluronate-binding protein, GHAP, ERVR

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

30599-1-PBS targets VCAN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen VCAN fusion protein Ag30151 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name versican
Synonyms PG M, Large fibroblast proteoglycan, Glial hyaluronate-binding protein, GHAP, ERVR
Calculated Molecular Weight 373 kDa
Observed Molecular Weight250-300 kDa, 550 kDa
GenBank Accession NumberNM_004385
Gene Symbol VCAN
Gene ID (NCBI) 1462
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13611
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Versican, a component of the extracellular matrix and a member of the lectican family of proteoglycans, is the largest chondroitin sulfate proteoglycan and has an important role in the development of the heart, musculoskeletal system and central nervous system (CNS). The structure of versican is characterized by an approximately 550-kDa core protein consisting of an amino-terminal (G1) hyaluronan binding domain, a carboxy-terminal (G3) domain, and two central chondroitin sulfate glycosaminoglycan attachment domains, the α-GAG and β-GAG domains. Versican has four different isoforms that differ in the central GAG attachment domain: V0 (contains both α and β GAG domains), V1 (contains only the β domain), V2 (contains only the α domain) and V3 (devoid of both GAG domains)(PMID: 26385570).