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验证数据展示

  • IF Staining of HUVEC using CL488-83766-2

    IF Staining of HUVEC using CL488-83766-2

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CoraLite® Plus 488 VE-cadherin/CD144 antibody (CL488-83766-2, Clone: 240755D8 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated VE-cadherin/CD144 Recombinant antibody

VE-cadherin/CD144 Recombinant Antibody for IF/ICC
Cat No. CL488-83766-2
CloneNo. 240755D8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHUVEC cells
For other applications, we recommend the unconjugated version of this antibody, 83766-2-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83766-2 targets VE-cadherin/CD144 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cadherin 5, type 2 (vascular endothelium)
Synonyms VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen
Calculated Molecular Weight88 kDa
Observed Molecular Weight130 kDa, 100 kDa
GenBank Accession NumberNM_001795.5
Gene Symbol VE-cadherin
Gene ID (NCBI) 1003
RRIDAB_3673302
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP33151-1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 VE-cadherin/CD144 antibody CL488-83766-2Download protocol
Standard Protocols
Click here to view our Standard Protocols