• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Mouse VEGFR2/KDR Recombinant antibody, PBS Only (Capture)

VEGFR2/KDR Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240214D7

Cat no : 83366-1-PBS

Print datasheet

Synonyms

VEGFR-2/Kdr, Kdr, Krd 1, Flk1, Flk 1

验证数据展示

  • WB analysis of mouse lung using 83366-1-RR (same clone as 83366-1-PBS)

    WB analysis of mouse lung using 83366-1-RR (same clone as 83366-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 83366-1-RR (VEGFR2/KDR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83366-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00394-1

    Cytometric bead array standard curve of MP00394-1

    Cytometric bead array standard curve of MP00394-1, MOUSE VEGFR2/KDR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83366-1-PBS. Detection antibody: 83366-3-PBS. Standard: Eg0703. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00394-3

    Cytometric bead array standard curve of MP00394-3

    Cytometric bead array standard curve of MP00394-3, MOUSE VEGFR2/KDR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83366-1-PBS. Detection antibody: 83366-2-PBS. Standard: Eg0703. Range: 5-80 ng/mL.

  • Affinity and Kinetic Characterization of 83366-1-RR

    Affinity and Kinetic Characterization of 83366-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83366-1-RR against Mouse VEGFR2/KDR were performed. The affinity constant is 1.88 nM.

产品信息

83366-1-PBS targets VEGFR2/KDR as part of a matched antibody pair:

MP00394-1: 83366-1-PBS capture and 83366-3-PBS detection (validated in Cytometric bead array)

MP00394-3: 83366-1-PBS capture and 83366-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name kinase insert domain protein receptor
Synonyms VEGFR-2/Kdr, Kdr, Krd 1, Flk1, Flk 1
Calculated Molecular Weight150KD
Observed Molecular Weight150-190 kDa
GenBank Accession NumberNM_010612.2
Gene Symbol VEGFR-2
Gene ID (NCBI) 16542
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35918-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VEGFR2, also named CD309, KDR, and FLK1, is a receptor for VEGF or VEGFC. VEGFR2 which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In the case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. VEGFR2 functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis, and sprouting.