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验证数据展示

  • IF Staining of human placenta using CL488-24849

    IF Staining of human placenta using CL488-24849

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite® Plus 488 VISTA antibody (CL488-24849) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human placenta using CL488-24849

    IF Staining of human placenta using CL488-24849

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite® Plus 488 VISTA antibody (CL488-24849) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CoraLite® Plus 488-conjugated VISTA Polyclonal antibody

VISTA Polyclonal Antibody for IF-P
Cat No. CL488-24849

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF-P

别名

B7-H5, C10orf54, GI24, Platelet receptor Gi24, PP2135, SISP1, UNQ730/PRO1412, VISTA, VSIR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman placenta tissue
Planning an IHC experiment? We recommend our IHCeasy VISTA Ready-To-Use IHC Kit. VISTA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 24849-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24849 targets VISTA in IF-P applications and shows reactivity with human samples.

经测试应用 IF-P Application Description
经测试反应性 human
免疫原 VISTA fusion protein Ag20600 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 10 open reading frame 54
别名 B7-H5, C10orf54, GI24, Platelet receptor Gi24, PP2135, SISP1, UNQ730/PRO1412, VISTA, VSIR
计算分子量 311 aa, 34 kDa
观测分子量45-60 kDa
GenBank蛋白编号BC020568
基因名称 VISTA
Gene ID (NCBI) 64115
RRIDAB_3672776
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H7M9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H and SISP1, is a member of the immunoglobulin superfamily. It serves as not only as a positive regulator but also as a substrate of MT1-MMP, and contributes at least in part to tumor invasion.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 VISTA antibody CL488-24849Download protocol
Standard Protocols
Click here to view our Standard Protocols