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CoraLite®532-conjugated VISTA Polyclonal antibody

VISTA Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF-P

Conjugate

CoraLite®532 Fluorescent Dye

Cat no : CL532-24849

Print datasheet

Synonyms

验证数据展示

  • IF Staining of human placenta using CL532-24849

    IF Staining of human placenta using CL532-24849

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite®532 VISTA antibody (CL532-24849) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human placenta using CL532-24849

    IF Staining of human placenta using CL532-24849

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite®532 VISTA antibody (CL532-24849) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF-P detected inhuman placenta tissue
Planning an IHC experiment? We recommend our IHCeasy VISTA Ready-To-Use IHC Kit. VISTA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 24849-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL532-24849 targets VISTA in IF-P applications and shows reactivity with human samples.

Tested Applications IF-P Application Description
Tested Reactivity human
Immunogen VISTA fusion protein Ag20600 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 10 open reading frame 54
Synonyms
Calculated Molecular Weight 311 aa, 34 kDa
Observed Molecular Weight45-60 kDa
GenBank Accession NumberBC020568
Gene Symbol VISTA
Gene ID (NCBI) 64115
Conjugate CoraLite®532 Fluorescent Dye
Excitation/Emission Maxima Wavelengths537 nm / 560 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H7M9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H and SISP1, is a member of the immunoglobulin superfamily. It serves as not only as a positive regulator but also as a substrate of MT1-MMP, and contributes at least in part to tumor invasion.

实验方案

Product Specific Protocols
IF protocol for CL532 VISTA antibody CL532-24849Download protocol
Standard Protocols
Click here to view our Standard Protocols