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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • IHC staining of human breast cancer using 66682-1-Ig (same clone as 66682-1-PBS)

    IHC staining of human breast cancer using 66682-1-Ig (same clone as 66682-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VWF antibody included
  • IHC staining of human breast cancer using 66682-1-Ig (same clone as 66682-1-PBS)

    IHC staining of human breast cancer using 66682-1-Ig (same clone as 66682-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VWF antibody included
  • IF Staining of human breast cancer using 66682-1-Ig (same clone as 66682-1-PBS)

    IF Staining of human breast cancer using 66682-1-Ig (same clone as 66682-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using VWF antibody (66682-1-Ig, Clone: 3F9F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 66682-1-Ig (same clone as 66682-1-PBS)

    IF Staining of human breast cancer using 66682-1-Ig (same clone as 66682-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using VWF antibody (66682-1-Ig, Clone: 3F9F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66682-1-Ig (same clone as 66682-1-PBS)

    IHC staining of human prostate cancer using 66682-1-Ig (same clone as 66682-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66682-1-Ig (VWF antibody) at dilution of 1:2000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with . This data was developed using the same antibody clone with 66682-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VWF antibody included

VWF Monoclonal antibody, PBS Only

VWF Monoclonal Antibody for IHC, IF-P, Indirect ELISA
Cat No. 66682-1-PBS

Print datasheet

CloneNo. 3F9F3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF-P, Indirect ELISA

Synonyms

VWF, VWFpp, VWD, von Willebrand antigen II, F8VWF, 3F9F3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy VWF Ready-To-Use IHC Kit. VWF primary antibody included.

产品信息

66682-1-PBS targets VWF in IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human
Immunogen VWF fusion protein Ag25578 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name von Willebrand factor
Synonyms VWF, VWFpp, VWD, von Willebrand antigen II, F8VWF, 3F9F3
Gene Symbol VWF
Gene ID (NCBI) 7450
RRIDAB_2882036
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Von Willebrand factor (VWF) is a large multimeric glycoprotein found in blood plasma involved in hemostasis following vascular injury. Due to the multimeric nature of VWF, it can range in size from 500 to 20,000 kDa due to the differences in the number of subunits comprising the protein. Each subunit is approximately 250 kDa (PMID: 9759493). The biosynthesis of VWF in vivo is limited to endothelial cells (PMID: 4209883) and megakaryocytes (PMID: 2413071). VWF synthesized in endothelial cells is either released directly into the plasma via 27186a secretory pathway, or tubulized and stored in organelles unique to this cell type called Weibel-Palade bodies (PMID: 16459301). Whereas VWF synthesized in megakaryocytes is stored in the alpha granules of platelets (PMID: 2046403). The primary function of VWF is as an adhesive plasma glycoprotein, particularly factor VIII; an essential blood-clotting protein (PMID: 6982084). VWF is also important in platelet adhesion to wound sites by binding specifically to type I and type III collagen (PMID: 11098050), with larger VWF multimers being most effective (PMID: 24448155).