VWF Monoclonal antibody

VWF Monoclonal Antibody for IHC, IF-P, ELISA
Cat No. 66682-1-Ig

产品说明书

CloneNo. 3F9F3

宿主/亚型

Mouse / IgG1

种属反应性

human and More (2)

应用

IHC, IF-P, ELISA and More (1)

VWF, VWFpp, VWD, von Willebrand antigen II, F8VWF, 3F9F3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -


经过测试的应用

Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human prostate cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue
Planning an IHC experiment? We recommend our IHCeasy VWF Ready-To-Use IHC Kit. VWF primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66682-1-Ig targets VWF in IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF-P, ELISA Application Description
文献引用应用IHC, IF
经测试反应性 human
文献引用反应性human, mouse, rat
免疫原 VWF fusion protein Ag25578 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 von Willebrand factor
别名 VWF, VWFpp, VWD, von Willebrand antigen II, F8VWF, 3F9F3
基因名称 VWF
Gene ID (NCBI) 7450
RRIDAB_2882036
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Von Willebrand factor (VWF) is a large multimeric glycoprotein found in blood plasma involved in hemostasis following vascular injury. Due to the multimeric nature of VWF, it can range in size from 500 to 20,000 kDa due to the differences in the number of subunits comprising the protein. Each subunit is approximately 250 kDa (PMID: 9759493). The biosynthesis of VWF in vivo is limited to endothelial cells (PMID: 4209883) and megakaryocytes (PMID: 2413071). VWF synthesized in endothelial cells is either released directly into the plasma via 27186a secretory pathway, or tubulized and stored in organelles unique to this cell type called Weibel-Palade bodies (PMID: 16459301). Whereas VWF synthesized in megakaryocytes is stored in the alpha granules of platelets (PMID: 2046403). The primary function of VWF is as an adhesive plasma glycoprotein, particularly factor VIII; an essential blood-clotting protein (PMID: 6982084). VWF is also important in platelet adhesion to wound sites by binding specifically to type I and type III collagen (PMID: 11098050), with larger VWF multimers being most effective (PMID: 24448155).

实验方案

Product Specific Protocols
IHC protocol for VWF antibody 66682-1-IgDownload protocol
IF protocol for VWF antibody 66682-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

J Cell Mol Med

Impact of chronic intermittent hypoxia on the long non-coding RNA and mRNA expression profiles in myocardial infarction.

Authors - Chaowei Hu
ratIF

Int J Stem Cells

Integrin α4 Positive Subpopulation in Adipose Derived Stem Cells Effectively Reduces Infarct Size through Enhanced Engraftment into Myocardial Infarction

Authors - Zihui Yuan
IF

Exp Ther Med

Biological activity of a vascular endothelial cell-hydroxyapatite orbital implant complex: An experimental study.

Authors - Weiqi Wu
mouseIF

Cell Death Dis

Association of N6-methyladenine DNA with plaque progression in atherosclerosis via myocardial infarction-associated transcripts.

Authors - Lianpin Wu
mouseIF

Inflammation

Low-Shear Stress Promotes Atherosclerosis via Inducing Endothelial Cell Pyroptosis Mediated by IKKε/STAT1/NLRP3 Pathway

Authors - Yifei Lv
rat,mouseIF

Int J Biol Sci

Metallothionein 3 Potentiates Pulmonary Artery Smooth Muscle Cell Proliferation by Promoting Zinc-MTF1-ATG5 Axis-mediated Autophagosome Formation

Authors - Tianxin Xiong
Loading...