验证数据展示
经过测试的应用
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue, human prostate cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human breast cancer tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 4 publications below |
| IF | See 12 publications below |
产品信息
66682-1-Ig targets VWF in IHC, IF-P, ELISA applications and shows reactivity with human samples.
| Tested Applications | IHC, IF-P, ELISA Application Description |
| Cited Applications | IHC, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Immunogen | VWF fusion protein Ag25578 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | von Willebrand factor |
| Synonyms | VWF, VWFpp, VWD, von Willebrand antigen II, F8VWF, 3F9F3 |
| Gene Symbol | VWF |
| Gene ID (NCBI) | 7450 |
| RRID | AB_2882036 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Von Willebrand factor (VWF) is a large multimeric glycoprotein found in blood plasma involved in hemostasis following vascular injury. Due to the multimeric nature of VWF, it can range in size from 500 to 20,000 kDa due to the differences in the number of subunits comprising the protein. Each subunit is approximately 250 kDa (PMID: 9759493). The biosynthesis of VWF in vivo is limited to endothelial cells (PMID: 4209883) and megakaryocytes (PMID: 2413071). VWF synthesized in endothelial cells is either released directly into the plasma via 27186a secretory pathway, or tubulized and stored in organelles unique to this cell type called Weibel-Palade bodies (PMID: 16459301). Whereas VWF synthesized in megakaryocytes is stored in the alpha granules of platelets (PMID: 2046403). The primary function of VWF is as an adhesive plasma glycoprotein, particularly factor VIII; an essential blood-clotting protein (PMID: 6982084). VWF is also important in platelet adhesion to wound sites by binding specifically to type I and type III collagen (PMID: 11098050), with larger VWF multimers being most effective (PMID: 24448155).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for VWF antibody 66682-1-Ig | Download protocol |
| IF protocol for VWF antibody 66682-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Mol Med Impact of chronic intermittent hypoxia on the long non-coding RNA and mRNA expression profiles in myocardial infarction. | ||
Int J Stem Cells Integrin α4 Positive Subpopulation in Adipose Derived Stem Cells Effectively Reduces Infarct Size through Enhanced Engraftment into Myocardial Infarction | ||
Exp Ther Med Biological activity of a vascular endothelial cell-hydroxyapatite orbital implant complex: An experimental study. | ||
Cell Death Dis Association of N6-methyladenine DNA with plaque progression in atherosclerosis via myocardial infarction-associated transcripts. | ||
Inflammation Low-Shear Stress Promotes Atherosclerosis via Inducing Endothelial Cell Pyroptosis Mediated by IKKε/STAT1/NLRP3 Pathway | ||
Int J Biol Sci Metallothionein 3 Potentiates Pulmonary Artery Smooth Muscle Cell Proliferation by Promoting Zinc-MTF1-ATG5 Axis-mediated Autophagosome Formation |
