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CoraLite®594-conjugated VWF Monoclonal antibody

VWF Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3F9F3

Cat no : CL594-66682

Print datasheet

Synonyms

F8VWF, von Willebrand antigen II, von Willebrand factor, VWD, VWF

验证数据展示

  • IF Staining of human breast cancer using CL594-66682

    IF Staining of human breast cancer using CL594-66682

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 vwf antibody (CL594-66682, Clone: 3F9F3 ) at dilution of 1:200.

  • IF Staining of human breast cancer using CL594-66682

    IF Staining of human breast cancer using CL594-66682

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 vwf antibody (CL594-66682, Clone: 3F9F3 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman breast cancer tissue
Planning an IHC experiment? We recommend our IHCeasy VWF Ready-To-Use IHC Kit. VWF primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66682-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66682 targets VWF in IF-P applications and shows reactivity with Human samples.

Tested Applications IF-P Application Description
Tested Reactivity Human
Immunogen VWF fusion protein Ag25578 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name von Willebrand factor
Synonyms F8VWF, von Willebrand antigen II, von Willebrand factor, VWD, VWF
Gene Symbol VWF
Gene ID (NCBI) 7450
RRIDAB_2920021
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Von Willebrand factor (VWF) is a large multimeric glycoprotein found in blood plasma involved in hemostasis following vascular injury. Due to the multimeric nature of VWF, it can range in size from 500 to 20,000 kDa due to the differences in the number of subunits comprising the protein. Each subunit is approximately 250 kDa (PMID: 9759493). The biosynthesis of VWF in vivo is limited to endothelial cells (PMID: 4209883) and megakaryocytes (PMID: 2413071). VWF synthesized in endothelial cells is either released directly into the plasma via 27186a secretory pathway, or tubulized and stored in organelles unique to this cell type called Weibel-Palade bodies (PMID: 16459301). Whereas VWF synthesized in megakaryocytes is stored in the alpha granules of platelets (PMID: 2046403). The primary function of VWF is as an adhesive plasma glycoprotein, particularly factor VIII; an essential blood-clotting protein (PMID: 6982084). VWF is also important in platelet adhesion to wound sites by binding specifically to type I and type III collagen (PMID: 11098050), with larger VWF multimers being most effective (PMID: 24448155).

实验方案

Product Specific Protocols
IF protocol for CL594 VWF antibody CL594-66682Download protocol
Standard Protocols
Click here to view our Standard Protocols