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验证数据展示

  • WB analysis using CL750-66305

    WB analysis using CL750-66305

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-66305 (Vinculin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CoraLite® Plus 750-conjugated Vinculin Monoclonal antibody

Vinculin Monoclonal Antibody for WB
Cat No. CL750-66305

Print datasheet

CloneNo. 2B5A7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB

Synonyms

VCL, MVCL, MV, Metavinculin, CMD1W

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 750
Size: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, PC-3 cells
For other applications, we recommend the unconjugated version of this antibody, 66305-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-66305 targets Vinculin in WB applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Vinculin fusion protein Ag24946 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vinculin
Synonyms VCL, MVCL, MV, Metavinculin, CMD1W
Calculated Molecular Weight 1133 aa, 124 kDa
Observed Molecular Weight 117 kDa
GenBank Accession NumberBC039174
Gene Symbol Vinculin
Gene ID (NCBI) 7414
RRIDAB_3084892
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP18206
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 Vinculin antibody CL750-66305Download protocol
Standard Protocols
Click here to view our Standard Protocols