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验证数据展示

  • WB analysis using 66305-1-Ig (same clone as 66305-1-PBS)

    WB analysis using 66305-1-Ig (same clone as 66305-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66305-1-Ig (same clone as 66305-1-PBS)

    IHC staining of human breast cancer using 66305-1-Ig (same clone as 66305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66305-1-Ig (same clone as 66305-1-PBS)

    IHC staining of human breast cancer using 66305-1-Ig (same clone as 66305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66305-1-Ig (same clone as 66305-1-PBS)

    IF Staining of HeLa using 66305-1-Ig (same clone as 66305-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66305-1-Ig(Vinculin antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66305-1-Ig (same clone as 66305-1-PBS)

    FC experiment of HeLa using 66305-1-Ig (same clone as 66305-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Vinculin Monoclonal antibody (66305-1-Ig, Clone:2B5A7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

Vinculin Monoclonal antibody, PBS Only

Vinculin Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66305-1-PBS

产品说明书

CloneNo. 2B5A7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

VCL, MVCL, MV, Metavinculin, CMD1W

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66305-1-PBS targets Vinculin in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Vinculin fusion protein Ag24946 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 vinculin
别名 VCL, MVCL, MV, Metavinculin, CMD1W
计算分子量 1133 aa, 124 kDa
观测分子量 117 kDa
GenBank蛋白编号BC039174
基因名称 Vinculin
Gene ID (NCBI) 7414
RRIDAB_2810300
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP18206
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.