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WDR21C Recombinant antibody, PBS Only (Detector)

WDR21C Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240402H3

Cat no : 83802-4-PBS

Print datasheet

Synonyms

WD repeat-containing protein 21C, WD repeat domain 21C, DDB1- and CUL4-associated factor 4-like protein 2, DCAF4L2, 240402H3

验证数据展示

  • WB analysis using 83802-4-RR (same clone as 83802-4-PBS)

    WB analysis using 83802-4-RR (same clone as 83802-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83802-4-RR (WDR21C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83802-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00738-2

    Cytometric bead array standard curve of MP00738-2

    Cytometric bead array standard curve of MP00738-2, WDR21C Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83802-1-PBS. Detection antibody: 83802-4-PBS. Standard: Ag30748. Range: 1.56-100 ng/mL.

  • Affinity and Kinetic Characterization of 83802-4-RR

    Affinity and Kinetic Characterization of 83802-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83802-4-RR against Human WDR21C were performed. The affinity constant is 0.479 nM.

产品信息

83802-4-PBS targets WDR21C as part of a matched antibody pair:

MP00738-2: 83802-1-PBS capture and 83802-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen WDR21C fusion protein Ag30748 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name WD repeat domain 21C
Synonyms WD repeat-containing protein 21C, WD repeat domain 21C, DDB1- and CUL4-associated factor 4-like protein 2, DCAF4L2, 240402H3
Calculated Molecular Weight 395 aa, 44 kDa
Observed Molecular Weight44-50 kDa
GenBank Accession NumberBC111778
Gene Symbol WDR21C
Gene ID (NCBI) 138009
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NA75
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WDR21C (WD repeat-containing protein 21C), also known as DCAF4L2. It is expected to be located in the cytoplasm, which is enriched in testis. WDR21C is a member of WD-repeat proteins, which commonly serve as mediators of protein-protein interplay. It is reported that elevated WDR21C expression in human colorectal cancer (CRC) significantly correlated with a more advanced clinical stage as in lymphatic and distant metastasis. More importantly, elevated WDR21C expression is an independent prognosis factor for survival (PMID: 27158335). The molecular weight of WDR21C is 43 kDa.