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  • Featured Product
  • KD/KO Validated

YES1 Monoclonal antibody, PBS Only

YES1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A12E7

Cat no : 67196-1-PBS

Print datasheet

Synonyms

c yes, HsT441, P61 YES, Proto oncogene c Yes, Yes, YES1

验证数据展示

  • WB analysis using 67196-1-Ig (same clone as 67196-1-PBS)

    WB analysis using 67196-1-Ig (same clone as 67196-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67196-1-Ig (YES1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67196-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 67196-1-Ig (same clone as 67196-1-PBS)

    WB analysis of HepG2 using 67196-1-Ig (same clone as 67196-1-PBS)

    WB result of YES1 antibody (67196-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-YES1 transfected HepG2 cells. This data was developed using the same antibody clone with 67196-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 67196-1-Ig (same clone as 67196-1-PBS)

    IHC staining of human kidney using 67196-1-Ig (same clone as 67196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67196-1-Ig (YES1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67196-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YES1 antibody included
  • IHC staining of human kidney using 67196-1-Ig (same clone as 67196-1-PBS)

    IHC staining of human kidney using 67196-1-Ig (same clone as 67196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67196-1-Ig (YES1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67196-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YES1 antibody included
Planning an IHC experiment? We recommend our IHCeasy YES1 Ready-To-Use IHC Kit. YES1 primary antibody included.

产品信息

67196-1-PBS targets YES1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, rat
Immunogen YES1 fusion protein Ag5016 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
Synonyms c yes, HsT441, P61 YES, Proto oncogene c Yes, Yes, YES1
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC048960
Gene Symbol YES1
Gene ID (NCBI) 7525
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07947
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

YES1, also named as p61-Yes and YES, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. YES1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.