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  • Featured Product
  • KD/KO Validated

YES1 Monoclonal antibody

YES1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A12E7

Cat no : 67196-1-Ig

Print datasheet

Synonyms

c yes, HsT441, P61 YES, Proto oncogene c Yes, Yes, YES1

验证数据展示

  • WB analysis using 67196-1-Ig

    WB analysis using 67196-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67196-1-Ig (YES1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 67196-1-Ig

    WB analysis of HepG2 using 67196-1-Ig

    WB result of YES1 antibody (67196-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-YES1 transfected HepG2 cells.

  • IHC staining of human kidney using 67196-1-Ig

    IHC staining of human kidney using 67196-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67196-1-Ig (YES1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with YES1 antibody included
  • IHC staining of human kidney using 67196-1-Ig

    IHC staining of human kidney using 67196-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67196-1-Ig (YES1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with YES1 antibody included

经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, HeLa cells, LNCaP cells, A431 cells, HSC-T6 cells, PC-12 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy YES1 Ready-To-Use IHC Kit. YES1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67196-1-Ig targets YES1 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, rat
Immunogen YES1 fusion protein Ag5016 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
Synonyms c yes, HsT441, P61 YES, Proto oncogene c Yes, Yes, YES1
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC048960
Gene Symbol YES1
Gene ID (NCBI) 7525
RRIDAB_2918483
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07947
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

YES1, also named as p61-Yes and YES, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. YES1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

实验方案

Product Specific Protocols
WB protocol for YES1 antibody 67196-1-IgDownload protocol
IHC protocol for YES1 antibody 67196-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols