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验证数据展示

  • FC experiment of HepG2 using CL488-67196

    FC experiment of HepG2 using CL488-67196

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human YES1 (CL488-67196, Clone:1A12E7) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated YES1 Monoclonal antibody

YES1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67196

产品说明书

CloneNo. 1A12E7

宿主/亚型

Mouse / IgG2a

种属反应性

human, rat

应用

FC (Intra)

别名

p61-Yes, P61 YES, HsT441, EC:2.7.10.2, c yes

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy YES1 Ready-To-Use IHC Kit. YES1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67196-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67196 targets YES1 in FC (Intra) applications and shows reactivity with human, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, rat
免疫原 YES1 fusion protein Ag5016 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
别名 p61-Yes, P61 YES, HsT441, EC:2.7.10.2, c yes
计算分子量 61 kDa
观测分子量 62 kDa
GenBank蛋白编号BC048960
基因名称 YES1
Gene ID (NCBI) 7525
RRIDAB_2923764
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07947
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YES1, also named as p61-Yes and YES, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. YES1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 YES1 antibody CL488-67196Download protocol
Standard Protocols
Click here to view our Standard Protocols