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验证数据展示

  • FC experiment of HepG2 using CL488-67196

    FC experiment of HepG2 using CL488-67196

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human YES1 (CL488-67196, Clone:1A12E7) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated YES1 Monoclonal antibody

YES1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67196

Print datasheet

CloneNo. 1A12E7

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

FC (Intra)

Synonyms

p61-Yes, P61 YES, HsT441, EC:2.7.10.2, c yes

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy YES1 Ready-To-Use IHC Kit. YES1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67196-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67196 targets YES1 in FC (Intra) applications and shows reactivity with human, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, rat
Immunogen YES1 fusion protein Ag5016 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
Synonyms p61-Yes, P61 YES, HsT441, EC:2.7.10.2, c yes
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC048960
Gene Symbol YES1
Gene ID (NCBI) 7525
RRIDAB_2923764
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07947
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YES1, also named as p61-Yes and YES, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. YES1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 YES1 antibody CL488-67196Download protocol
Standard Protocols
Click here to view our Standard Protocols