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YY1 Recombinant antibody, PBS Only

YY1 Uni-rAbTM Recombinant Antibody for IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2K12

Cat no : 82712-5-PBS

Print datasheet

Synonyms

NF-E1, INO80S, INO 80S, Delta transcription factor, DELTA

验证数据展示

  • IF Staining of HepG2 using 82712-5-RR (same clone as 82712-5-PBS)

    IF Staining of HepG2 using 82712-5-RR (same clone as 82712-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (82712-5-RR, Clone: 2K12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82712-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82712-5-RR (same clone as 82712-5-PBS)

    IF Staining of HepG2 using 82712-5-RR (same clone as 82712-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (82712-5-RR, Clone: 2K12 ) at dilution of 1:700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82712-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82712-5-RR

    Affinity and Kinetic Characterization of 82712-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82712-5-RR against Human YY1 were performed. The affinity constant is 0.412 pM.

Planning an IHC experiment? We recommend our IHCeasy YY1 Ready-To-Use IHC Kit. YY1 primary antibody included.

产品信息

82712-5-PBS targets YY1 in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen YY1 fusion protein Ag17792 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name YY1 transcription factor
Synonyms NF-E1, INO80S, INO 80S, Delta transcription factor, DELTA
Calculated Molecular Weight 414 aa, 45 kDa
GenBank Accession NumberBC037308
Gene Symbol YY1
Gene ID (NCBI) 7528
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25490
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.