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验证数据展示

  • IF Staining of HepG2 using 82712-5-RR

    IF Staining of HepG2 using 82712-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (82712-5-RR, Clone: 2K12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IF Staining of HepG2 using 82712-5-RR

    IF Staining of HepG2 using 82712-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (82712-5-RR, Clone: 2K12 ) at dilution of 1:700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 82712-5-RR

    FC experiment of HeLa using 82712-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug YY1 Recombinant antibody (82712-5-RR, Clone:2K12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82712-5-RR

    Affinity and Kinetic Characterization of 82712-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82712-5-RR against Human YY1 were performed. The affinity constant is 0.412 pM.

YY1 Recombinant antibody

YY1 Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 82712-5-RR

产品说明书

CloneNo. 2K12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

NF-E1, INO80S, INO 80S, Delta transcription factor, DELTA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy YY1 Ready-To-Use IHC Kit. YY1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:350-1:1400
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82712-5-RR targets YY1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 YY1 fusion protein Ag17792 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 YY1 transcription factor
别名 NF-E1, INO80S, INO 80S, Delta transcription factor, DELTA
计算分子量 414 aa, 45 kDa
GenBank蛋白编号BC037308
基因名称 YY1
Gene ID (NCBI) 7528
RRIDAB_3670536
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25490
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.

实验方案

Product Specific Protocols
IF protocol for YY1 antibody 82712-5-RRDownload protocol
FC protocol for YY1 antibody 82712-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols