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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68282-1-Ig (same clone as 68282-1-PBS)

    WB analysis using 68282-1-Ig (same clone as 68282-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68282-1-Ig (ZC3H8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68282-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 68282-1-Ig (same clone as 68282-1-PBS)

    FC experiment of HEK-293 using 68282-1-Ig (same clone as 68282-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug ZC3H8 Monoclonal antibody (68282-1-Ig, Clone:1G8C6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 68282-1-PBS in a different storage buffer formulation.

ZC3H8 Monoclonal antibody, PBS Only

ZC3H8 Monoclonal Antibody for Indirect ELISA, WB
Cat No. 68282-1-PBS

产品说明书

CloneNo. 1G8C6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

Indirect ELISA, WB

别名

Zinc finger CCCH domain-containing protein 8, ZC3HDC8, Fliz1, 1G8C6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68282-1-PBS targets ZC3H8 in Indirect ELISA, WB applications and shows reactivity with human samples.

经测试应用 Indirect ELISA, WB Application Description
经测试反应性 human
免疫原 ZC3H8 fusion protein Ag23496 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 zinc finger CCCH-type containing 8
别名 Zinc finger CCCH domain-containing protein 8, ZC3HDC8, Fliz1, 1G8C6
观测分子量36 kDa
GenBank蛋白编号BC032001
基因名称 ZC3H8
Gene ID (NCBI) 84524
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8N5P1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZC3H8, also named as Fliz1, is a novel zinc finger protein preferentially expressed in fetal liver hematopoietic progenitors and in several adult organs, including the thymus. It acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene. Overexpression of Fliz1 repressed the expression of GATA-3 in vivo and in vitro. GATA-3 might be partly regulated by the intronic regulatory region and Fliz1 in a developmental stage-specific fashion (PMID: 23932780).