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ZC3H8 Monoclonal antibody, PBS Only

ZC3H8 Monoclonal Antibody for Indirect ELISA, WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

1G8C6

Cat no : 68282-1-PBS

Print datasheet

Synonyms

Zinc finger CCCH domain-containing protein 8, ZC3HDC8, Fliz1, 1G8C6

验证数据展示

  • WB analysis using 68282-1-Ig (same clone as 68282-1-PBS)

    WB analysis using 68282-1-Ig (same clone as 68282-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68282-1-Ig (ZC3H8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68282-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 68282-1-Ig (same clone as 68282-1-PBS)

    FC experiment of HEK-293 using 68282-1-Ig (same clone as 68282-1-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug ZC3H8 Monoclonal antibody (68282-1-Ig, Clone:1G8C6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 68282-1-PBS in a different storage buffer formulation.

产品信息

68282-1-PBS targets ZC3H8 in Indirect ELISA, WB applications and shows reactivity with human samples.

Tested Applications Indirect ELISA, WB Application Description
Tested Reactivity human
Immunogen ZC3H8 fusion protein Ag23496 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name zinc finger CCCH-type containing 8
Synonyms Zinc finger CCCH domain-containing protein 8, ZC3HDC8, Fliz1, 1G8C6
Observed Molecular Weight36 kDa
GenBank Accession NumberBC032001
Gene Symbol ZC3H8
Gene ID (NCBI) 84524
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8N5P1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZC3H8, also named as Fliz1, is a novel zinc finger protein preferentially expressed in fetal liver hematopoietic progenitors and in several adult organs, including the thymus. It acts as a transcriptional repressor of the GATA3 promoter. Sequence-specific DNA-binding factor that binds to the 5'-AGGTCTC-3' sequence within the negative cis-acting element intronic regulatory region (IRR) of the GATA3 gene. Overexpression of Fliz1 repressed the expression of GATA-3 in vivo and in vitro. GATA-3 might be partly regulated by the intronic regulatory region and Fliz1 in a developmental stage-specific fashion (PMID: 23932780).