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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 750-conjugated ZO-2 Recombinant antibody

ZO-2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230474D1

Cat no : CL750-83111

Print datasheet

Synonyms

TJP2, ZO2, TJP 2, Tight junction protein ZO-2, Tight junction protein ZO 2

验证数据展示

  • WB analysis using CL750-83111

    WB analysis using CL750-83111

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-83111 (ZO-2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using CL750-83111

    FC experiment of A431 using CL750-83111

    1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated ZO-2 Recombinant antibody (CL750-83111, Clone:230474D1)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells
Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 83111-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-83111 targets ZO-2 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen ZO-2 fusion protein Ag13534 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tight junction protein 2 (zona occludens 2)
Synonyms TJP2, ZO2, TJP 2, Tight junction protein ZO-2, Tight junction protein ZO 2
Calculated Molecular Weight 134 kDa
Observed Molecular Weight ~150 kDa
GenBank Accession NumberBC027592
Gene Symbol TJP2
Gene ID (NCBI) 9414
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UDY2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 ZO-2 antibody CL750-83111Download protocol
FC protocol for CL Plus 750 ZO-2 antibody CL750-83111Download protocol
Standard Protocols
Click here to view our Standard Protocols