CoraLite® Plus 750-conjugated ZO-2 Recombinant antibody

ZO-2 Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230474D1

Cat No : CL750-83111

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Synonyms

Tight junction protein 2, Tight junction protein ZO 2, TJP2, X104, ZO 2, ZO2, Zona occludens protein 2, Zonula occludens protein 2



经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 83111-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-83111 targets ZO-2 in WB applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB Application Description
Tested Reactivity Human, mouse, rat
Immunogen ZO-2 fusion protein Ag13534 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tight junction protein 2 (zona occludens 2)
Synonyms Tight junction protein 2, Tight junction protein ZO 2, TJP2, X104, ZO 2, ZO2, Zona occludens protein 2, Zonula occludens protein 2
Calculated Molecular Weight 134 kDa
Observed Molecular Weight ~150 kDa
GenBank Accession NumberBC027592
Gene Symbol TJP2
Gene ID (NCBI) 9414
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UDY2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 ZO-2 antibody CL750-83111Download protocol
Standard Protocols
Click here to view our Standard Protocols