验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, MCF-7 cells |
| Positive FC (Intra) detected in | A431 cells |
For other applications, we recommend the unconjugated version of this antibody, 83111-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-83111 targets ZO-2 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | ZO-2 fusion protein Ag13534 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | tight junction protein 2 (zona occludens 2) |
| 别名 | TJP2, ZO2, TJP 2, Tight junction protein ZO-2, Tight junction protein ZO 2 |
| 计算分子量 | 134 kDa |
| 观测分子量 | ~150 kDa |
| GenBank蛋白编号 | BC027592 |
| 基因名称 | TJP2 |
| Gene ID (NCBI) | 9414 |
| RRID | AB_3673769 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9UDY2 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 ZO-2 antibody CL750-83111 | Download protocol |
| FC protocol for CL Plus 750 ZO-2 antibody CL750-83111 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |