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ChromoTek Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 647 [CTK0101, CTK0102]

Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH is an anti-human IgG and anti-rabbit IgG specific secondary antibody. It consists of of a mixture of 2 Nanobodies that bind to human IgG and rabbit IgG with high affinity & specificity.

Applications

IF, WB, FC

Reactivity

Rabbit, Human, Macaque

Host

Alpaca

Conjugate

Alexa Fluor® 647

Cat No : srbAF647-1

Synonyms

human IgG, rabbit IgG, immunoglobulin Gamma

Validation Data Gallery

  • Well-defined and characterized immunostaining: Primary anti-rabbit IgG antibody (grey) with 2 copies each of a rabbit Fab- and Fc-specific monoclonal Nanobodies (green) bound. In total, 8 fluorophores (red stars) label the primary rabbit IgG antibody.

    Well-defined and characterized immunostaining: Primary anti-rabbit IgG antibody (grey) with 2 copies each of a rabbit Fab- and Fc-specific monoclonal Nanobodies (green) bound. In total, 8 fluorophores (red stars) label the primary rabbit IgG antibody.

  • HeLa cells stably expressing Tubulin-GFP at near-endogenous level were immunostained with rabbit anti-GFP PABG1 antibody and alpaca anti-rabbit IgG VHH Alexa Fluor® 647 (magenta). Nuclei were detected with H2B-RFP and RFP-Booster Atto594 (cyan). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    HeLa cells stably expressing Tubulin-GFP at near-endogenous level were immunostained with rabbit anti-GFP PABG1 antibody and alpaca anti-rabbit IgG VHH Alexa Fluor® 647 (magenta). Nuclei were detected with H2B-RFP and RFP-Booster Atto594 (cyan). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • Higher resolution with anti-rabbit IgG Nano-Secondaries compared to conventional secondary antibodies: Left: Formation of a small, precise complex of Nanobodies (green) & primary antibody (grey). Right: Formation of a large, arbitrary complex of multiple polyclonal secondaries (green) & primary rabbit antibody.

    Higher resolution with anti-rabbit IgG Nano-Secondaries compared to conventional secondary antibodies: Left: Formation of a small, precise complex of Nanobodies (green) & primary antibody (grey). Right: Formation of a large, arbitrary complex of multiple polyclonal secondaries (green) & primary rabbit antibody.

  • HeLa cells were immunostained with rabbit anti-Lamin B1 antibodies and alpaca anti-rabbit IgG VHH Alexa Fluor® 568 (1:1,000). Confocal and gated STED images were acquired with a Leica TCS SP8 STED 3X microscope, pulsed depletion with a 775 nm laser. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    HeLa cells were immunostained with rabbit anti-Lamin B1 antibodies and alpaca anti-rabbit IgG VHH Alexa Fluor® 568 (1:1,000). Confocal and gated STED images were acquired with a Leica TCS SP8 STED 3X microscope, pulsed depletion with a 775 nm laser. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • One-step immunostaining (top row) vs. sequential immunostaining (bottom row) of HeLa cells. Anti-β-Actin (left column) and anti-LaminB1 (right column) primary rabbit antibodies + secondary alpaca anti-rabbit IgG VHH Alexa Fluor® 647. Scale bar, 20 μm.

    One-step immunostaining (top row) vs. sequential immunostaining (bottom row) of HeLa cells. Anti-β-Actin (left column) and anti-LaminB1 (right column) primary rabbit antibodies + secondary alpaca anti-rabbit IgG VHH Alexa Fluor® 647. Scale bar, 20 μm.

Product Information

Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH is an anti-human IgG and anti-rabbit IgG specific secondary antibody. It consists of of a mixture of 2 Nanobodies that bind to human IgG and rabbit IgG with high affinity & specificity.

DescriptionNano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH reagent uses a novel class of anti-rabbit IgG and anti-human IgG specific antibodies. This secondary antibody product consists of Nanobodies that bind to rabbit IgG and human IgG with high affinity & specificity. Nano-Secondary reagents enable simultaneous incubation with primary antibodies, thus shortening your experimental. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below.
ApplicationsIF, WB, FC
HostAlpaca
Species ReactivityRabbit, Human, Macaque
No cross-reactivity to mouse, rat, sheep, goat, and guinea pig IgG
Target/SpecificityFc-fragment of human IgG, Fab- and Fc-fragment of rabbit IgG (co-reactivity)
Physical StateLiquid
ConjugateAlexa Fluor® 647
Suggested DilutionImmunofluorescence 1:1,000
Super-resolution microscopy 1:1,000
Western blot 1:1,000
Purification MethodRecombinant expression, affinity purification IMAC
TypeMixture of 2 monoclonal Nanobodies; Secondary Nanobody
ClassRecombinant
FormatAlpaca single domain antibody, monovalent
Cross-reactivityNo cross-reactivity to goat, guinea pig, mouse, rat, and sheep serum
Cross-reactivity to macaque serum
ImmunogenPurified rabbit IgG
ClonalityBiclonal: mixture of 2 monoclonal Nanobodies
ClonesCTK0101 (VHH0244): Fc-fragment of human IgG, Fc-fragment of rabbit IgG
CTK0102 (VHH0245): Fab-fragment of rabbit IgG
Affinity (KD)CTK0101: KD = 0.2 nM, CTK0102: KD = 1.2 nM
RRIDAB_2827587
Excitation / EmissionExcitation max: 650 nm, Emission max: 665 nm
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
Storage ConditionStore at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles.
Size10 µL; 100 µL
NoteThis product is for research use only, not for diagnostic or therapeutic use

Publications

ApplicationTitle
IF

Biosens Bioelectron

3D co-culture of macrophages and fibroblasts in a sessile drop array for unveiling the role of macrophages in skin wound-healing

Authors - Xiaoyan Lyu
View Article
IF

Vet Microbiol

NDV inhibited IFN-β secretion through impeding CHCHD10-mediated mitochondrial fusion to promote viral proliferation

Authors - Xibing Yu
View Article
IF

Nat Methods

Spatial single-cell mass spectrometry defines zonation of the hepatocyte proteome

Authors - Florian A Rosenberger
View Article