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ChromoTek Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 568 [CTK0101, CTK0102]

Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH is an anti-human IgG and anti-rabbit IgG specific secondary antibody. It consists of of a mixture of 2 Nanobodies that bind to human IgG and rabbit IgG with high affinity & specificity.

Applications

IF, WB, FC

Reactivity

Rabbit, Human, Macaque

Host

Alpaca

Conjugate

Alexa Fluor® 568

Cat No : srbAF568-1

Synonyms

human IgG, rabbit IgG, immunoglobulin Gamma

Validation Data Gallery

  • Well-defined and characterized immunostaining: Primary anti-rabbit IgG antibody (grey) with 2 copies each of a rabbit Fab- and Fc-specific monoclonal Nanobodies (green) bound. In total, 8 fluorophores (red stars) label the primary rabbit IgG antibody.

    Well-defined and characterized immunostaining: Primary anti-rabbit IgG antibody (grey) with 2 copies each of a rabbit Fab- and Fc-specific monoclonal Nanobodies (green) bound. In total, 8 fluorophores (red stars) label the primary rabbit IgG antibody.

  • Higher resolution with anti-rabbit IgG Nano-Secondaries compared to conventional secondary antibodies: Left: Formation of a small, precise complex of Nanobodies (green) & primary antibody (grey). Right: Formation of a large, arbitrary complex of multiple polyclonal secondaries (green) & primary rabbit antibody.

    Higher resolution with anti-rabbit IgG Nano-Secondaries compared to conventional secondary antibodies: Left: Formation of a small, precise complex of Nanobodies (green) & primary antibody (grey). Right: Formation of a large, arbitrary complex of multiple polyclonal secondaries (green) & primary rabbit antibody.

  • Alpaca anti-rabbit IgG VHH Alexa Fluor® 568 was applied together with rabbit anti-Ki67 antibodies for detection of Ki67 (red) in HeLa cells. Scale bar, 20 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Alpaca anti-rabbit IgG VHH Alexa Fluor® 568 was applied together with rabbit anti-Ki67 antibodies for detection of Ki67 (red) in HeLa cells. Scale bar, 20 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • HeLa cells were immunostained with rabbit anti-Lamin B1 antibodies and alpaca anti-rabbit IgG VHH Alexa Fluor® 568 (1:1,000). Confocal and gated STED images were acquired with a Leica TCS SP8 STED 3X microscope, pulsed depletion with a 775 nm laser. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    HeLa cells were immunostained with rabbit anti-Lamin B1 antibodies and alpaca anti-rabbit IgG VHH Alexa Fluor® 568 (1:1,000). Confocal and gated STED images were acquired with a Leica TCS SP8 STED 3X microscope, pulsed depletion with a 775 nm laser. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • HeLa cells were immunostained with rabbit anti-Lamin B1 antibodies and alpaca anti-rabbit IgG VHH Alexa Fluor® 568 (1:1,000). Confocal and gated STED images were acquired with a Leica TCS SP8 STED 3X microscope, pulsed depletion with a 775 nm laser. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    HeLa cells were immunostained with rabbit anti-Lamin B1 antibodies and alpaca anti-rabbit IgG VHH Alexa Fluor® 568 (1:1,000). Confocal and gated STED images were acquired with a Leica TCS SP8 STED 3X microscope, pulsed depletion with a 775 nm laser. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Product Information

Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH is an anti-human IgG and anti-rabbit IgG specific secondary antibody. It consists of of a mixture of 2 Nanobodies that bind to human IgG and rabbit IgG with high affinity & specificity.

DescriptionNano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH reagent uses a novel class of anti-rabbit IgG and anti-human IgG specific antibodies. This secondary antibody product consists of Nanobodies that bind to rabbit IgG and human IgG with high affinity & specificity. Nano-Secondary reagents enable simultaneous incubation with primary antibodies, thus shortening your experimental. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below.
ApplicationsIF, WB, FC
HostAlpaca
Species ReactivityRabbit, Human, Macaque
No cross-reactivity to mouse, rat, sheep, goat, and guinea pig IgG
Target/SpecificityFc-fragment of human IgG, Fab- and Fc-fragment of rabbit IgG (co-reactivity)
Physical StateLiquid
ConjugateAlexa Fluor® 568
Suggested DilutionImmunofluorescence 1:1,000
Super-resolution microscopy 1:1,000
Western blot 1:1,000
Purification MethodRecombinant expression, affinity purification IMAC
TypeMixture of 2 monoclonal Nanobodies; Secondary Nanobody
ClassRecombinant
FormatAlpaca single domain antibody, monovalent
Cross-reactivityNo cross-reactivity to goat, guinea pig, mouse, rat, and sheep serum
Cross-reactivity to macaque serum
ImmunogenPurified rabbit IgG
ClonalityBiclonal: mixture of 2 monoclonal Nanobodies
ClonesCTK0101 (VHH0244): Fc-fragment of human IgG, Fc-fragment of rabbit IgG
CTK0102 (VHH0245): Fab-fragment of rabbit IgG
Affinity (KD)CTK0101: KD = 0.2 nM, CTK0102: KD = 1.2 nM
RRIDAB_2827586
Excitation / EmissionExcitation max: 578 nm, Emission max: 603 nm
Storage Buffer10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
Storage ConditionStore at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles.
Size10 µL; 100 µL
NoteThis product is for research use only, not for diagnostic or therapeutic use

Publications

ApplicationTitle
IF

Sci Adv

Mutation of SLC7A14 causes auditory neuropathy and retinitis pigmentosa mediated by lysosomal dysfunction.

Authors - Kimberlee P Giffen
View Article
IF

Gene Ther

A new protocol for whole-brain biodistribution analysis of AAVs by tissue clearing, light-sheet microscopy and semi-automated spatial quantification

Authors - Miguel M Lopes
View Article
IF

Neuroscience

Translocator Protein Regulate Polarization Phenotype Transformation of Microglia after Cerebral Ischemia-reperfusion Injury

Authors - Shadamu Yusuying
View Article
IF

Front Bioeng Biotechnol

Maintenance of multipotency of bone marrow mesenchymal stem cells on poly(ε-caprolactone) nanoneedle arrays through the enhancement of cell-cell interaction

Authors - Xiaoxue Ren
View Article
IF

Phytomedicine

Hesperidin inhibits lung fibroblast senescence via IL-6/STAT3 signaling pathway to suppress pulmonary fibrosis

Authors - Di Han
View Article
IF

EMBO Rep

Myddosome clustering in IL-1 receptor signaling regulates the formation of an NF-kB activating signalosome

Authors - Fakun Cao
View Article