• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82869-1-RR

    WB analysis using 82869-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82869-1-RR (cGAS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HAP1 using 82869-1-RR

    WB analysis of HAP1 using 82869-1-RR

    WB result of cGAS antibody (82869-1-RR; 1:1000; room temperature for 1.5 hours) with wild-type and cGAS knockout HAP1 cells.

  • FC experiment of A431 using 82869-1-RR

    FC experiment of A431 using 82869-1-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug cGAS Recombinant antibody (82869-1-RR, Clone:1H1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of A431 using 82869-1-RR

    IF Staining of A431 using 82869-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using cGAS antibody (82869-1-RR, Clone: 1H1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of A431 using 82869-1-RR

    IF Staining of A431 using 82869-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using cGAS antibody (82869-1-RR, Clone: 1H1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 82869-1-RR

    Affinity and Kinetic Characterization of 82869-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82869-1-RR against Human cGAS were performed. The affinity constant is 0.124 nM.

  • Featured Product
  • KD/KO验证

cGAS Recombinant antibody

cGAS Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82869-1-RR

产品说明书

CloneNo. 1H1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

h-cGAS, EC:2.7.7.86, cGAMP synthase, C6orf150, 2'3'-cGAMP synthase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HAP1 cells, HL-60 cells, THP-1 cells, MCF-7 cells, NK-92 cells
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82869-1-RR targets cGAS in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 cGAS fusion protein Ag23455 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 6 open reading frame 150
别名 h-cGAS, EC:2.7.7.86, cGAMP synthase, C6orf150, 2'3'-cGAMP synthase
计算分子量 496 aa, 54 kDa
观测分子量62 kDa
GenBank蛋白编号BC113608
基因名称 cGAS
Gene ID (NCBI) 115004
RRIDAB_3670589
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N884
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.

实验方案

Product Specific Protocols
WB protocol for cGAS antibody 82869-1-RRDownload protocol
IF protocol for cGAS antibody 82869-1-RRDownload protocol
FC protocol for cGAS antibody 82869-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols