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Host
0
Species Reactivity
0
Species Reactivity
0
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0
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0
Conjugate
0

验证数据展示

  • WB analysis using 82869-1-RR (same clone as 82869-1-PBS)

    WB analysis using 82869-1-RR (same clone as 82869-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82869-1-RR (cGAS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82869-1-PBS in a different storage buffer formulation.

  • WB analysis of HAP1 using 82869-1-RR (same clone as 82869-1-PBS)

    WB analysis of HAP1 using 82869-1-RR (same clone as 82869-1-PBS)

    WB result of cGAS antibody (82869-1-RR; 1:1000; room temperature for 1.5 hours) with wild-type and cGAS knockout HAP1 cells. This data was developed using the same antibody clone with 82869-1-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 82869-1-RR (same clone as 82869-1-PBS)

    FC experiment of A431 using 82869-1-RR (same clone as 82869-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug cGAS Recombinant antibody (82869-1-RR, Clone:1H1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82869-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 82869-1-RR (same clone as 82869-1-PBS)

    IF Staining of A431 using 82869-1-RR (same clone as 82869-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using cGAS antibody (82869-1-RR, Clone: 1H1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82869-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 82869-1-RR (same clone as 82869-1-PBS)

    IF Staining of A431 using 82869-1-RR (same clone as 82869-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using cGAS antibody (82869-1-RR, Clone: 1H1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82869-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82869-1-RR

    Affinity and Kinetic Characterization of 82869-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82869-1-RR against Human cGAS were performed. The affinity constant is 0.124 nM.

  • Featured Product
  • KD/KO验证

cGAS Recombinant antibody, PBS Only

cGAS Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 82869-1-PBS

产品说明书

CloneNo. 1H1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

h-cGAS, EC:2.7.7.86, cGAMP synthase, C6orf150, 2'3'-cGAMP synthase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82869-1-PBS targets cGAS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 cGAS fusion protein Ag23455 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 6 open reading frame 150
别名 h-cGAS, EC:2.7.7.86, cGAMP synthase, C6orf150, 2'3'-cGAMP synthase
计算分子量 496 aa, 54 kDa
观测分子量62 kDa
GenBank蛋白编号BC113608
基因名称 cGAS
Gene ID (NCBI) 115004
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8N884
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs.